Document Detail


In vitro and in vivo antitumor activity of a methanol extract of Dregea volubilis leaves with its antioxidant effect.
MedLine Citation:
PMID:  22239161     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONTEXT: In India, Dregea volubilis (L.f.) Benth. ex Hook.f. (Asclepediaceae), a large twining shrub with a woody vine, is used to treat tumors traditionally.
OBJECTIVE: This study evaluated the in vitro and in vivo antitumor activity of the methanol extract of Dregea volubilis leaves (MEDV) and elucidated its possible mechanism of action.
MATERIALS AND METHODS: In vitro antitumor activity of MEDV was evaluated against Ehrlich ascites carcinoma (EAC) cell-line. In vivo antitumor and antioxidant activity of MEDV at three dose levels (50, 100, and 200 mg/kg) were determined against EAC tumor-bearing mice. After 24 h of EAC inoculation, the extract was administered for 9 consecutive days. After the administration of the last dose on the 9th day followed by 18 h fasting, mice from all groups were sacrificed to determine antitumor activity and hematological profiles along with liver related biochemical parameters like lipid peroxidation, antioxidant enzymatic activity, etc.
RESULTS: For in vitro antitumor activity, IC(50) value of MEDV for EAC tumor cells was 85.51 ± 4.07 µg/ml. The MEDV showed a decrease in tumor volume, packed cell volume and viable cell count and an increase in the non-viable cell count of the EAC tumor-bearing mice (p < 0.001). Hematological profile reverted near to normal level in extract treated mice. MEDV decreased the hepatic lipid peroxidation level and enhanced superoxide dismutase and catalase level in tumor-bearing mice (p < 0.001).
DISCUSSION AND CONCLUSION: MEDV exhibited in vitro and in vivo antitumor activity in EAC tumor-bearing mice mediated through augmenting antioxidant defense system.
Authors:
Emdad Hossain; Saikat Chakroborty; Ahmed Milan; Pronobesh Chattopadhyay; Subhash C Mandal; J K Gupta
Publication Detail:
Type:  Journal Article     Date:  2012-01-13
Journal Detail:
Title:  Pharmaceutical biology     Volume:  50     ISSN:  1744-5116     ISO Abbreviation:  Pharm Biol     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-10     Completed Date:  2012-05-31     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  9812552     Medline TA:  Pharm Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  338-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Phytogenic / administration & dosage,  isolation & purification,  pharmacology*
Antioxidants / administration & dosage,  isolation & purification,  pharmacology*
Asclepiadaceae / chemistry*
Carcinoma, Ehrlich Tumor / drug therapy,  pathology
Catalase / metabolism
Dose-Response Relationship, Drug
Female
India
Inhibitory Concentration 50
Lipid Peroxidation / drug effects
Male
Medicine, Ayurvedic
Mice
Plant Extracts / administration & dosage,  pharmacology*
Plant Leaves
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Antioxidants; 0/Plant Extracts; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase

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