| In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures. | |
| | |
MedLine Citation:
|
PMID: 19594888 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
ABSTRACT: BACKGROUND: Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb Arnebia euchroma (Royle) Johnst. METHODS: The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the in vitro antitumor effects of acetylshikonin on human lung adenocarcinoma cell line A549, human hepatocellular carcinoma cell line Bel-7402, human breast adenocarcinoma cell line MCF-7 and mouse Lewis lung carcinoma (LLC) cell line. C57BL/6 mice with LLC model were used to study the in vivo antitumor effects of acetylshikonin. The expression of bax, bcl-2 and caspase-3 proteins in LLC tissue was determined with immunohistochemical staining. RESULTS: In A549, Bel-7402, MCF-7 and LLC cell lines, acetylshikonin inhibited cell growth in a dose-dependent manner. IC50 (means +/- SD) were 5.6 +/- 0.86 mug/ml, 6.82 +/- 1.5 mug/ml, 3.04 +/- 0.44 mug/ml and 2.72 +/- 0.38 mug/ml respectively. Acetylshikonin suppressed tumor growth in C57BL/6 mice with LLC. The inhibition rate of acetylshikonin (2 mg/kg) was 42.85%. Immunohistochemical staining revealed that in the acetylshikonin groups the expression of bax and caspase-3 increased, whereas the expression of bcl-2 decreased, suggesting that acetylshikonin induced tumor cell apoptosis through activating the pro-apoptotic bcl-2 family and caspase-3. CONCLUSION: Acetylshikonin isolated from Arnebia euchroma (Royle) Johnst cell suspension cultures exhibits specific in vivo and in vitro antitumor effects. |
| | |
Authors:
|
Wenbi Xiong; Gang Luo; Liming Zhou; Yun Zeng; Wenji Yang |
Related Documents
:
|
9615758 - Dolastatin 15 induces apoptosis and bcl-2 phosphorylation in small cell lung cancer cel... 14710848 - The presence of 19-kda bcl-2 in dividing cells. 16465208 - Bcl-2 prevents activation of cpp32 cysteine protease and cleavage of poly (adp-ribose) ... 12408758 - Overexpression of bax up-regulates the sensitivity of hcc-9204 cell apoptosis induced b... 971588 - The influence of different freezing procedures and different cryoprotective agents on t... 22175298 - L-arginine is a radioprotector for hematopoietic progenitor cells. |
Publication Detail:
|
Type: Journal Article Date: 2009-07-11 |
Journal Detail:
|
Title: Chinese medicine Volume: 4 ISSN: 1749-8546 ISO Abbreviation: - Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-07-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101265109 Medline TA: Chin Med Country: England |
Other Details:
|
Languages: eng Pagination: 14 Citation Subset: - |
Affiliation:
|
Department of Pharmacology, West China Center for Medical Sciences, Sichuan University, Chengdu, Sichuan 610041, PR China. zhou108@163.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Energy profile and secondary structure impact shRNA efficacy.
Next Document: Offense related characteristics and psychosexual development of juvenile sex offenders.