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In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures.
MedLine Citation:
PMID:  19594888     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT: BACKGROUND: Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb Arnebia euchroma (Royle) Johnst. METHODS: The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the in vitro antitumor effects of acetylshikonin on human lung adenocarcinoma cell line A549, human hepatocellular carcinoma cell line Bel-7402, human breast adenocarcinoma cell line MCF-7 and mouse Lewis lung carcinoma (LLC) cell line. C57BL/6 mice with LLC model were used to study the in vivo antitumor effects of acetylshikonin. The expression of bax, bcl-2 and caspase-3 proteins in LLC tissue was determined with immunohistochemical staining. RESULTS: In A549, Bel-7402, MCF-7 and LLC cell lines, acetylshikonin inhibited cell growth in a dose-dependent manner. IC50 (means +/- SD) were 5.6 +/- 0.86 mug/ml, 6.82 +/- 1.5 mug/ml, 3.04 +/- 0.44 mug/ml and 2.72 +/- 0.38 mug/ml respectively. Acetylshikonin suppressed tumor growth in C57BL/6 mice with LLC. The inhibition rate of acetylshikonin (2 mg/kg) was 42.85%. Immunohistochemical staining revealed that in the acetylshikonin groups the expression of bax and caspase-3 increased, whereas the expression of bcl-2 decreased, suggesting that acetylshikonin induced tumor cell apoptosis through activating the pro-apoptotic bcl-2 family and caspase-3. CONCLUSION: Acetylshikonin isolated from Arnebia euchroma (Royle) Johnst cell suspension cultures exhibits specific in vivo and in vitro antitumor effects.
Authors:
Wenbi Xiong; Gang Luo; Liming Zhou; Yun Zeng; Wenji Yang
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Publication Detail:
Type:  Journal Article     Date:  2009-07-11
Journal Detail:
Title:  Chinese medicine     Volume:  4     ISSN:  1749-8546     ISO Abbreviation:  -     Publication Date:  2009  
Date Detail:
Created Date:  2009-07-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265109     Medline TA:  Chin Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  14     Citation Subset:  -    
Affiliation:
Department of Pharmacology, West China Center for Medical Sciences, Sichuan University, Chengdu, Sichuan 610041, PR China. zhou108@163.com.
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