Document Detail


In vitro and in situ expression of IL-23 by keratinocytes in healthy skin and psoriasis lesions: enhanced expression in psoriatic skin.
MedLine Citation:
PMID:  16424222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Keratinocytes contribute to cutaneous immune responses through the expression of cytokines. We investigated whether human keratinocytes can express IL-23, a newly defined IFN-gamma-inducing cytokine composed of a unique p19 subunit and a p40 subunit shared with IL-12. Cultured keratinocytes from normal and lesional psoriatic skin were found to express constitutively mRNA for both subunits of IL-23. Low but significant levels of the heterodimeric IL-23 protein could be detected in cell lysates and supernatants from stimulated keratinocytes by immunoblotting and ELISA. Functional analysis showed that these low levels of keratinocyte-derived IL-23 were sufficient to enhance the IFN-gamma production by memory T cells. Immunostaining of skin sections confirmed expression of both subunits of IL-23 by keratinocytes in situ and also revealed expression of this cytokine in the dermal compartment. IL-23 expression was significantly higher in psoriatic lesional skin, compared with normal and psoriatic nonlesional skin. The immunostained preparations of cultured cells and IL-23 levels in culture supernatants did not show any difference between normal and psoriatic keratinocytes indicating no intrinsic aberration of IL-23 expression in keratinocytes from psoriatic skin. Double staining of cytospin preparations demonstrated that IL-23 p19 is also expressed by epidermal Langerhans cells, dermal dendritic cells, and macrophages. Psoriasis is a chronic inflammatory skin disease mediated by IFN-gamma-expressing type 1 memory T cells. As IL-23 is important to activate memory T cells to produce IFN-gamma, its augmented expression of IL-23 by keratinocytes and cutaneous APC may contribute to the perpetuation of the inflammation process in this disease.
Authors:
Gamze Piskin; Regien M R Sylva-Steenland; Jan D Bos; Marcel B M Teunissen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  176     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-20     Completed Date:  2006-03-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1908-15     Citation Subset:  AIM; IM    
Affiliation:
Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. g.piskin@amc.uva.nl
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic / biosynthesis*,  genetics
Antigen-Presenting Cells / immunology,  metabolism,  pathology
Cells, Cultured
Dermis / immunology,  metabolism,  pathology
Dimerization
Epidermis / immunology,  metabolism,  pathology
Humans
Immunologic Memory / physiology
Inflammation Mediators / metabolism,  physiology
Interferon-gamma / biosynthesis
Interleukin-23
Interleukin-23 Subunit p19
Interleukins / biosynthesis*,  genetics
Keratinocytes / immunology*,  metabolism*,  pathology
Psoriasis / immunology*,  metabolism,  pathology
RNA, Messenger / metabolism
Skin / immunology*,  metabolism*
T-Lymphocytes / metabolism
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/IL23A protein, human; 0/Inflammation Mediators; 0/Interleukin-23; 0/Interleukin-23 Subunit p19; 0/Interleukins; 0/RNA, Messenger; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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