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In vitro immunohistochemical localization of S-phase cells by a monoclonal antibody to bromodeoxyuridine.
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MedLine Citation:
PMID:  2639694     Owner:  NLM     Status:  MEDLINE    
Bromodeoxyuridine, an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase of the cell cycle. In vitro immunohistochemical application of the BrdU/anti-BrdU-MAb method permits a quantitative assessment of the proliferative activity of a tissue as well as the direct location of the actively replicating cells in histological sections. In this paper, a method for the detection of the labeling index of S-phase cells in normal and neoplastic tissues with in vitro BrdU labeling and standard immunohistochemical techniques using anti-BrdU-MAb and avidin-biotin peroxidase complex is described. We have employed this method in 47 human solid tumor samples, including squamous cell carcinomas of head and neck and cervix uteri, adenocarcinomas and malignant lymphomas, and also evaluated the possible application of the BrdU labeling index to estimate the cycling S-phase cells in neoplastic cell populations. In our data, the in vitro labeling index varied greatly in an individual case (3.56-29.2%) and from an area to an area within the same case. Squamous cell carcinomas of the head and neck showed higher LI than those of the cervix uteri. A case of metastatic carcinoma to the lung from ductal carcinoma of the breast had the highest LI (29.2%), in contrast to the low LI (3.6%) in the primary ductal carcinoma of breast.
S S Lee; T S Lee; J S Park; E K Cho; C H Chung
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Korean medical science     Volume:  4     ISSN:  1011-8934     ISO Abbreviation:  J. Korean Med. Sci.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-08-22     Completed Date:  1990-08-22     Revised Date:  2011-03-17    
Medline Journal Info:
Nlm Unique ID:  8703518     Medline TA:  J Korean Med Sci     Country:  KOREA    
Other Details:
Languages:  eng     Pagination:  193-201     Citation Subset:  IM    
Department of Pathology, Obstetrics and Gynecology, Keimyung University School of Medicine.
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MeSH Terms
Adenocarcinoma / immunology,  pathology
Antibodies, Monoclonal / immunology*
Breast Neoplasms / immunology,  pathology
Bromodeoxyuridine / immunology*
Carcinoma, Squamous Cell / immunology,  pathology
Cell Nucleus / immunology,  physiology*
Head and Neck Neoplasms / immunology,  pathology
Lymphoma / immunology,  pathology
Neoplasms / immunology,  pathology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Korean Med Sci
Journal ID (pmc): jkms
ISSN: 1011-8934
ISSN: 1598-6357
Publisher: Korean Academy of Medical Sciences
Article Information
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Print publication date: Month: 12 Year: 1989
Volume: 4 Issue: 4
First Page: 193 Last Page: 201
ID: 3053700
PubMed Id: 2639694

In vitro immunohistochemical localization of S-phase cells by a monoclonal antibody to bromodeoxyuridine.
S. S. Lee
T. S. Lee
J. S. Park
E. K. Cho
C. H. Chung
Department of Pathology, Obstetrics and Gynecology, Keimyung University School of Medicine.

Article Categories:
  • Research Article

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