| In vitro heparinization of canine whole blood with low molecular weight heparin (dalteparin) significantly and dose-dependently prolongs heparinase-modified tissue factor-activated thromboelastography parameters and prothrombinase-induced clotting time. | |
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MedLine Citation:
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PMID: 19055570 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Low-molecular-weight heparin (LMWH) is being used increasingly in veterinary medicine for both treatment and prophylaxis of thromboembolic disease, but no predictable patient-side method exists to monitor its effect. OBJECTIVES: The aim of this study was to evaluate thromboelastography (TEG) and prothombinase-induced clotting time (PiCT) assays for detecting hemostatic alterations following in vitro heparinization of canine whole blood with dalteparin (Fragmin). METHODS: Citrated whole-blood samples were collected from 7 clinically healthy dogs. Dalteparin was added at concentrations of 0, 0.156, 0.625, 1.25, and 2.5 U/mL of whole blood. TEG was performed using heparinase cups with tissue factor (TF, 1:50,000) and kaolin as activators. Reaction time (R), clotting time (K), angle (alpha), and maximum amplitude (MA) were recorded. PiCT and anti-FXa activity were measured in plasma. RESULTS: With TF, increasing concentrations of dalteparin significantly prolonged R and K and significantly decreased alpha and MA. K, alpha, and MA ratios were significantly different from baseline at all dalteparin concentrations and R was significantly different from baseline at concentrations of 0.625, 1.25, and 2.5 U/mL. With kaolin, only R was significantly different from baseline at dalteparin concentrations of 0.625 and 2.5 U/mL. PiCT detected dalteparin concentrations < or = 0.625 U/mL, with a good linear correlation (r(2)=.96, P<.0001). CONCLUSION: These results suggest that TF-activated TEG and PiCT assays should be further evaluated as promising new methods for evaluating the effect of LMWH, using doses in the recommended clinical range and prospective clinical studies. |
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Authors:
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Lisbeth R Jessen; Bo Wiinberg; Asger L Jensen; Mads Kjelgaard-Hansen; Kate H Jensen; Lotte B Pedersen; Annemarie T Kristensen |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Veterinary clinical pathology / American Society for Veterinary Clinical Pathology Volume: 37 ISSN: 0275-6382 ISO Abbreviation: Vet Clin Pathol Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-05 Completed Date: 2009-01-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9880575 Medline TA: Vet Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 363-72 Citation Subset: IM |
Affiliation:
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Department of Small Animal Clinical Sciences, Faculty of Life Sciences, University of Copenhagen, Denmark. lrmj@life.ku.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticoagulants / pharmacology Blood Coagulation / drug effects Dalteparin / pharmacology* Dogs Dose-Response Relationship, Drug Female Heparin Lyase / metabolism* Kaolin Male Thrombelastography / veterinary* Thromboplastin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Dalteparin; 1332-58-7/Kaolin; 9035-58-9/Thromboplastin; EC 4.2.2.7/Heparin Lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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