Document Detail

In vitro growth stimulatory and in vivo wound healing studies on cycloartane-type saponins of Astragalus genus.
MedLine Citation:
PMID:  21291980     Owner:  NLM     Status:  Publisher    
ETHNOPHARMACOLOGICAL RELEVANCE: Astragalus roots are used for their wound healing properties in Southeast Anatolia-Turkey. AIM OF THE STUDY: The present study was undertaken to evaluate the wound healing effects of the four chief saponins of Astragalus species [Cycloastragenol (CA), astragaloside IV (AG), cyclocephaloside I (CCI) and cyclocanthoside E (CCE)]. MATERIAL AND METHODS: Effects of cell viability and proliferation of the isolated compounds were evaluated by the MTT assay on human keratinocyte. The wound healing activity was studied by using in vitro wound healing, proliferation and migration scratch assay. In order to see in vivo effectiveness of the compounds, an animal study with Sprague-Dawley male rats at the age of 12 weeks was carried out, and then the main histological outcomes were investigated to observe reepithelization, neovascularization, and presence of inflammatory cells, granulation tissue amount and maturation. RESULTS: All the compounds increased both fibroblast proliferation and migration, but the effects were much superior for CA at 1ng/ml concentration. Among the compounds, based on the histological findings, 5% CA preparation was found to be the most remarkable in vivo wound healing agent showing greater cell density, more regularly organized dermis and more newly formed blood vessels CONCLUSION: Results of this study indicate that the cycloartane-type saponins are the principal constituents responsible for wound healing activities of the roots of Astragalus species substantiating its use in traditional medicine.
Canan Sevimli-Gür; Ilyas Onbaşılar; Pergin Atilla; Rükan Genç; Nur Cakar; Ismet Deliloğlu-Gürhan; Erdal Bedir
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-31
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  -     ISSN:  1872-7573     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-2-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ireland Ltd.
Department of Bioengineering, Engineering Faculty, Ege University, Bornova, 35100 İzmir, Turkey;; Kocaeli University, Science and Art Faculty, Department of Biology, Kocaeli, Turkey.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Tannin-Rich Fraction from Terminaliacatappa inhibits quorum sensing (QS) in Chromobacteriumviolaceum...
Next Document:  Ethnobotanical survey of medicinal plants commonly used by Kani tribals in Tirunelveli hills of West...