Document Detail


In vitro growth of TS/A adenocarcinoma and of the gene transfected TS/A-IL4 line on biological substrates.
MedLine Citation:
PMID:  10769654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The growth capacity and adaptation of TS/A and TS/A-IL4 lines on laminin, fibronectin, collagens I and IV and matrigel compared to plastics were studied by flow cytometry. On plastic plates, TS/A-IL4 grows in vitro more slowly than the TS/A line and shows a more differentiated phenotype. TS/A-IL4 cells loose the capacity to bind lymphocytes and peroxidase positive cells obtained from mice implanted with the same tumour. The ratio between fibroblast- and epithelial-like cells of TS/A adenocarcinoma is subjected to marked modifications depending on the substrate on which the two cell lines are grown. IL4 release per cell unit is increased by collagen I as is the number of CD54 positive cells, suggesting that, at least in part, the in vivo rejection of TS/A-IL4 tumor might be ascribed to the stimulatory effect of the tissue on the IL4 release by tumor cells. The overall result is that gene modified TS/A-IL4 line shows marked changes of behaviour, most of them depending on the substrate on which tumor cells are growing.
Authors:
S Pacor; M Magnarin; M E Carotenuto; P Spessotto; G Zabucchi; G Sava
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  20     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2000-05-11     Completed Date:  2000-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  191-6     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, University of Trieste, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Culture Techniques / instrumentation,  methods*
Cell Cycle
Cell Differentiation
Clone Cells / pathology,  secretion
Coculture Techniques
Collagen
Drug Combinations
Epithelial Cells / pathology
Female
Fibroblasts / pathology
Fibronectins
Graft Rejection
Intercellular Adhesion Molecule-1 / analysis
Interleukin-4 / genetics*,  secretion
Laminin
Lymphocyte Activation
Mammary Neoplasms, Experimental / genetics,  pathology*,  secretion
Mice
Mice, Inbred BALB C
Neoplasm Proteins / analysis
Neoplasm Transplantation
Plastics
Proteoglycans
Recombinant Fusion Proteins / secretion
Transfection
Tumor Cells, Cultured / pathology,  secretion
Chemical
Reg. No./Substance:
0/Drug Combinations; 0/Fibronectins; 0/Laminin; 0/Neoplasm Proteins; 0/Plastics; 0/Proteoglycans; 0/Recombinant Fusion Proteins; 119978-18-6/matrigel; 126547-89-5/Intercellular Adhesion Molecule-1; 207137-56-2/Interleukin-4; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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