| In vitro growth of TS/A adenocarcinoma and of the gene transfected TS/A-IL4 line on biological substrates. | |
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MedLine Citation:
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PMID: 10769654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The growth capacity and adaptation of TS/A and TS/A-IL4 lines on laminin, fibronectin, collagens I and IV and matrigel compared to plastics were studied by flow cytometry. On plastic plates, TS/A-IL4 grows in vitro more slowly than the TS/A line and shows a more differentiated phenotype. TS/A-IL4 cells loose the capacity to bind lymphocytes and peroxidase positive cells obtained from mice implanted with the same tumour. The ratio between fibroblast- and epithelial-like cells of TS/A adenocarcinoma is subjected to marked modifications depending on the substrate on which the two cell lines are grown. IL4 release per cell unit is increased by collagen I as is the number of CD54 positive cells, suggesting that, at least in part, the in vivo rejection of TS/A-IL4 tumor might be ascribed to the stimulatory effect of the tissue on the IL4 release by tumor cells. The overall result is that gene modified TS/A-IL4 line shows marked changes of behaviour, most of them depending on the substrate on which tumor cells are growing. |
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Authors:
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S Pacor; M Magnarin; M E Carotenuto; P Spessotto; G Zabucchi; G Sava |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 20 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2000 Jan-Feb |
Date Detail:
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Created Date: 2000-05-11 Completed Date: 2000-05-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: GREECE |
Other Details:
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Languages: eng Pagination: 191-6 Citation Subset: IM |
Affiliation:
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Department of Biomedical Sciences, University of Trieste, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Cell Culture Techniques / instrumentation, methods* Cell Cycle Cell Differentiation Clone Cells / pathology, secretion Coculture Techniques Collagen Drug Combinations Epithelial Cells / pathology Female Fibroblasts / pathology Fibronectins Graft Rejection Intercellular Adhesion Molecule-1 / analysis Interleukin-4 / genetics*, secretion Laminin Lymphocyte Activation Mammary Neoplasms, Experimental / genetics, pathology*, secretion Mice Mice, Inbred BALB C Neoplasm Proteins / analysis Neoplasm Transplantation Plastics Proteoglycans Recombinant Fusion Proteins / secretion Transfection Tumor Cells, Cultured / pathology, secretion |
| Chemical | |
Reg. No./Substance:
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0/Drug Combinations; 0/Fibronectins; 0/Laminin; 0/Neoplasm Proteins; 0/Plastics; 0/Proteoglycans; 0/Recombinant Fusion Proteins; 119978-18-6/matrigel; 126547-89-5/Intercellular Adhesion Molecule-1; 207137-56-2/Interleukin-4; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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