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In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions. Studies based on the acute neutropenia of filtration leukophoresis.
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MedLine Citation:
PMID:  894188     Owner:  NLM     Status:  MEDLINE    
To study mechanisms and mediators regulating the distribution of intravascular granulocytes between circulating and marginated pools, a human model with extreme transient margination, the neutropenia of continuous flow filtration leukophoresis, was analyzed. Studies in animals demonstrated the existence of a complement (C)-derived granulocytopenia-inducing factor. Thus, autologous plasma, exposed to nylon fibers (NF) of the filtration system, produced an acute selective decrement of circulating granulocytes and monocytes. This phenomenon was blocked by decomplementing plasma, by pretreatment of plasma with EDTA or hydrazine, and by preheating at 56 degrees C, but did occur after recombination of heat-inactivated and hydrazine-treated plasma before NF exposure. Preheating plasma at 50 degrees C did not inhibit the neutropenic response, suggesting involvement of the classical pathway of C activation. Ultrafiltration studies indicated that the NF-provoked neutropenia-inducing factor has a mol wt in the range of 10,000-30,000, and is heat stable (56 degrees C). To analyze the hypothesis that C- induced neutrophil margination might be consequent to increased cell adhesiveness to endothelial surfaces, the role of C in promoting granulocyte adherence was evaluated in vitro. Measured with a plastic Petridish assay, granulocyte adherence was significantly reduced in heat- inactivated (56 degrees C) and hydrazine-treated plasma, but adherence promoting capacity was restored by mixing the two plasmas, or by adding purified C3 to hydrazine-treated plasma. After exposure to activated C, neutrophils showed significantly increased adhesiveness which was maintained when cells were resuspended in heat-inactivated plasma, but progressively lost when resuspended in fresh plasma. On the basis of these results we conclude that granulocyte adhesiveness in vitro and margination in vivo are closely associated, C-dependent phenomena.
J Fehr; H S Jacob
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  146     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1977-10-20     Completed Date:  1977-10-20     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  641-52     Citation Subset:  AIM; IM    
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MeSH Terms
Agranulocytosis / blood*
Cell Adhesion*
Complement C3 / physiology
Complement System Proteins / physiology*
Granulocytes / immunology*
Hot Temperature
Hydrazines / pharmacology
Inulin / pharmacology
Leukocytes / immunology*
Neutropenia / blood*
Zymosan / pharmacology
Reg. No./Substance:
0/Complement C3; 0/Hydrazines; 9005-80-5/Inulin; 9007-36-7/Complement System Proteins; 9010-72-4/Zymosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 9 Year: 1977
Volume: 146 Issue: 3
First Page: 641 Last Page: 652
ID: 2180784
Publisher Id: 1463641
PubMed Id: 894188

In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions
J Fehr
HS Jacob

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