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In vitro induction/inhibition of germinal vesicle breakdown (GVBD) in frog (Euphlyctis cyanophlyctis) oocytes by endocrine active compounds.
MedLine Citation:
PMID:  22946474     Owner:  NLM     Status:  Publisher    
Oocyte maturation is transformation of oocytes into a fertilizable egg. This study examined the effects of four classes of chemicals: 1) acephate (organophosphate); 2) atrazine (herbicide); 3) cypermethrin and fenvalerate (synthetic pyrethroids); and 4) carbaryl (carbamate) on in vitro germinal vesicle breakdown (GVBD) of Euphlyctis cyanophlyctis oocytes. Follicles were isolated and defolliculated from surgically removed ovaries of E. cyanophlyctis and exposed to either progesterone (1 μM/mL) or graded concentrations (1, 5, 10, 15, and 20 μg/mL) of test chemicals. GVBD was evident by the presence of a white spot in the animal pole as well as the absence of germinal vesicles in sectioned heat-fixed oocytes. Percent GVBD was scored every 4 hours until 24 hours. Progesterone induced 77-84% GVBD, compared to 29-33% in controls, at 24 hours. Acephate induced 46-67% GVBD, whereas atrazine elicited 58-77% of GVBD. In cypermethrin or carbaryl- or fenvalerate-exposed oocytes, GVBD was limited to 22-28, 17-29 and 18-24%, respectively. The study infers that some chemical contaminants in the aquatic system may interfere with GVBD in amphibians. Because oocyte maturation is a prerequisite for the production of fertilizable eggs, any alteration in this process potentially impairs the fecundity of females.
Manjunath G Ghodageri; Pancharatna Katti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-5
Journal Detail:
Title:  Drug and chemical toxicology     Volume:  -     ISSN:  1525-6014     ISO Abbreviation:  Drug Chem Toxicol     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7801723     Medline TA:  Drug Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Zoology, Karnatak University , Dharwad , India.
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