Document Detail

In vitro genotoxicity and cytotoxicity in murine fibroblasts exposed to EDTA, NaOCl, MTAD and citric acid.
MedLine Citation:
PMID:  23306229     Owner:  NLM     Status:  In-Data-Review    
The aim of the present study was to evaluate the capacity of some root canal irrigants to induce genetic damage and/or cellular death in vitro. Murine fibroblast cells were exposed to ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl), MTAD™ and citric acid in increasing concentrations for 3 h at 37ºC. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 3 h at 37°C, and the positive control group was treated with methylmetanesulfonate, 1 μM. for 3 h at 37°C. Cytotoxicity was assessed by the trypan blue test and genotoxicity was evaluated by the single cell gel (comet) assay. The results showed that exposure to 2.5% and 5% NaOCl and 8.5% citric acid resulted in a significant cytotoxic effect. NaOCl, EDTA and citric acid did not produce genotoxic effects with respect to the comet assay data for all evaluated concentrations. Although MTAD was not a cytotoxic agent, it showed significant genotoxic effects at all tested concentrations (ANOVA and Tukey's test; p<0.05). NaOCl, EDTA and citric acid were found to be cytotoxic in a dose-dependent manner, but they were not genotoxic. MTAD did not cause cell death, but presented genotoxic effects.
Juliana Soares Roter Marins; Luciana Moura Sassone; Sandra Rivera Fidel; Daniel Araki Ribeiro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brazilian dental journal     Volume:  23     ISSN:  1806-4760     ISO Abbreviation:  Braz Dent J     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2013-01-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214652     Medline TA:  Braz Dent J     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  527-33     Citation Subset:  D    
Proclin Department, Dental School, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
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