| In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta). | |
| | |
MedLine Citation:
|
PMID: 21461639 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The origin of the myofibroblast, the primary effector cell of liver fibrosis, is still elusive. Here, we report that fluorescence-activated cell sorting purified E-cad + rhesus monkey liver epithelial progenitor cells (mLEPCs) may serve as a potential source for liver myofibroblasts. Adult mLEPCs colonies were cultured in medium containing 2 ng/ml transforming growth factor β (TGF-β) and 10% fetal bovine serum (FBS) to induce differentiation. Phenotypic changes of cells were analyzed by morphological observation, immunostaining, and reverse transcription-polymerase chain reaction (RT-PCR). After cultured with TGF-β and FBS, some cells in adult mLEPCs colonies converted to fibroblasts-like cells. Immunostaining showed that fibroblasts-like cells had acquired the expression of mesenchymal cell marker vimentin but lost the expression of epithelial cell marker CK8. Fibroblasts-like cells were maintained in culture for up to 40 passages. RT-PCR analysis revealed that fibroblasts-like cells had acquired the expression of mesenchymal genes (snail, PAI-1, and collagen I) and lost the expression of epithelial specific genes (E-cad, ZO-1, CK18, and occludin). In addition, more than 60% of fibroblasts-like cells expressed myofibroblastic-related proteins such as αSMA, vimentin, and N-cad, which were not presented in mLEPCs. Furthermore, increased cell motility was also detected in these fibroblasts-like cells by time-lapse video observation. Our results demonstrate that hepatic epithelial progenitor cells, mLEPCs, transform to myofibroblast-like cells via epithelial-mesenchymal transition. This finding will facilitate understanding of the origin of myofibroblasts in liver fibrosis. |
| | |
Authors:
|
Shaohui Ji; Xihong Wang; Jianhong Shu; Aijing Sun; Wei Si; Xiangyu Guo; Bo Zhao; Weizhi Ji; Lifang Jin |
Related Documents
:
|
19774649 - 5-aminosalicylic acid interferes in the cell cycle of colorectal cancer cells and induc... 19926479 - Healthy clocks, healthy body, healthy mind. 8390519 - Differential expression and activity of p34cdc2 in cultured aortic adventitial fibrobla... 18163989 - Retinoid processing in cone and müller cell lines. 9103229 - Unique cd8+ t cell-rich lymphoid aggregates in human uterine endometrium. 12176019 - Cell-cycle-dependent regulation of cnt1, a concentrative nucleoside transporter involve... 9059919 - The neural cell recognition molecule f11 is expressed on müller cells and schwann cell... 20873809 - High-performance photoelectrochemical cells from ionic liquid electrolyte in methyl-ter... 1670579 - Pcna/cyclin expression and brdu uptake define different subpopulations in different cel... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-4-1 |
Journal Detail:
|
Title: In vitro cellular & developmental biology. Animal Volume: - ISSN: 1543-706X ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-4-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
College of Life Science of Shaoxing University, 900# Chennan Dadao, Shaoxing, Zhejiang, 312000, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Unconditioned and conditioned effects of intranasally administered insulin vs placebo in healthy men...
Next Document: Acute kidney injury mediated by oxidative stress in Egyptian horses with exertional rhabdomyolysis.