Document Detail


In vitro fusion of newt macrophages.
MedLine Citation:
PMID:  3404109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spontaneous formation of multinucleate giant cells is often observed in in vitro cultures of peritoneal adherent macrophages from the newts, Notophthalmus viridescens and Taricha granulosa (urodele amphibians). The frequency of such giant cells in these cultures is increased by the addition of phorbol myristic acetate at the initiation of the cultures. This high frequency of multinucleate cells permitted us to evaluate whether multinucleate giant cells arise by cell fusion and/or by repeated nuclear division without cytokinesis. Cell fusion is readily detectable by scanning electron microscopy. To determine whether nuclear division without cytokinesis also occurs, some cultures were treated with colchicine to arrest mitotic figures; others were pulsed with tritiated thymidine to detect DNA synthesis. Mitotic figures were not seen in acridine orange-stained samples. In monolayers that were processed for autoradiography, only a few nuclei were marked with tritium. These observations suggest that nuclear division does not contribute significantly, if at all, to the formation of multinucleate giant cells from cultured newt peritoneal macrophages.
Authors:
B Płytycz; S W Potter; N Cohen; C J Bayne
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  246     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-09-09     Completed Date:  1988-09-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  319-23     Citation Subset:  IM    
Affiliation:
Department of Zoology, Oregon State University, Corvallis 97331.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Cell Fusion / drug effects
Cell Line
Colchicine / pharmacology
Female
Macrophages / drug effects,  physiology*
Male
Microscopy, Electron, Scanning
Notophthalmus viridescens / physiology*
Peritoneum / cytology
Salamandridae / physiology*
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
ID/Acronym/Agency:
HD 07901/HD/NICHD NIH HHS; HD 19530/HD/NICHD NIH HHS; RR 07079/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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