| In vitro flow evaluation of recombinant tissue factor pathway inhibitor immobilized on collagen impregnated Dacron. | |
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MedLine Citation:
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PMID: 15307537 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recombinant tissue factor pathway inhibitor (rTFPI) was immobilized on collagen impregnated (CI) knitted Dacron surfaces and its resistance to fibrin deposition evaluated following exposure to nonanticoagulated whole blood. Recombinant TFPI readily adsorbed to the CI Dacron surface and maintained its inhibitory activity. Under static conditions, rTFPI treated CI Dacron showed little fibrin deposition when compared with untreated surfaces. Treated samples exposed to flowing native blood at wall shear rates of 100 or 200 sec(-1) also demonstrated reduced fibrin deposition (up to 56%) compared with untreated samples. To assess the relative roles of the contact and tissue factor pathways in fibrin formation on artificial grafts, flow studies were performed with whole blood containing corn trypsin inhibitor, a potent inhibitor of FXIIa and contact activation. Corn trypsin inhibitor reduced fibrin deposition on untreated CI Dacron by 40%. Immobilized rTFPI alone, or corn trypsin inhibitor in combination with immobilized rTFPI, reduced fibrin deposition by 58% and 61%, respectively. These data suggest that immobilized rTFPI slows fibrin deposition on the vascular graft material by inhibiting both the contact pathway and blood borne tissue factor procoagulant activity arising from either the alternatively spliced form of tissue factor or from tissue factor containing microparticles. |
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Authors:
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Deepti A Raybagkar; Sirisha Patchipulusu; Alan E Mast; Connie L Hall |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: ASAIO journal (American Society for Artificial Internal Organs : 1992) Volume: 50 ISSN: 1058-2916 ISO Abbreviation: ASAIO J. Publication Date: 2004 Jul-Aug |
Date Detail:
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Created Date: 2004-08-13 Completed Date: 2004-10-19 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9204109 Medline TA: ASAIO J Country: United States |
Other Details:
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Languages: eng Pagination: 301-5 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, The University of Memphis, Memphis, TN, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption
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drug effects Blood Physiological Phenomena Blood Vessel Prosthesis* Coated Materials, Biocompatible* Collagen* Equipment Design Fibrin / metabolism Fibrinolytic Agents / administration & dosage*, pharmacology Hemorheology Humans Lipoproteins / administration & dosage*, pharmacology Microscopy, Electron, Scanning Polyethylene Terephthalates* Recombinant Proteins / administration & dosage, pharmacology Thrombosis / prevention & control |
| Chemical | |
Reg. No./Substance:
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0/Coated Materials, Biocompatible; 0/Fibrinolytic Agents; 0/Lipoproteins; 0/Polyethylene Terephthalates; 0/Recombinant Proteins; 0/lipoprotein-associated coagulation inhibitor; 9001-31-4/Fibrin; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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