Document Detail

In vitro fertilization and development of frozen-thawed bovine oocytes.
MedLine Citation:
PMID:  1395686     Owner:  NLM     Status:  MEDLINE    
Bovine oocytes were vitrified (V-oocytes) or frozen slowly (S-oocytes) at the germinal vesicle (GV) stage or after maturation in vitro (IVM) and their survival assessed morphologically and also by in vitro fertilization (IVF) and culture. The morphological survival of S-oocytes was 30.7% after freezing at the GV stage and 53.3% after IVM. The corresponding survival rates of V-oocytes were significantly lower, viz. 14.6 and 14.0%, respectively. The fertilization rate of S-oocytes frozen after IVM (51.0%) was lower than that of unfrozen controls (75.8%), but higher than after other treatments. Development continued in 16.0% of the fertilized S-oocytes, compared to 39.4% of control IVF zygotes and 1.6% developed into morulae or blastocysts (4.5% in controls). Only 0.8% of frozen-thawed GV stage oocytes and 4.6% of post-IVM V-oocytes cleaved after IVF and none formed morulae or blastocysts. Transfer of four embryos (two morulae and two blastocysts) derived from post-IVM S-oocytes into a recipient heifer resulted in pregnancy and the birth of twin calves.
E Fuku; T Kojima; Y Shioya; G J Marcus; B R Downey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryobiology     Volume:  29     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-11-13     Completed Date:  1992-11-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  485-92     Citation Subset:  IM    
Department of Animal Science, McGill University, Ste. Anne de Bellevue, Quebec, Canada.
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MeSH Terms
Cell Survival
Embryo Transfer
Embryonic and Fetal Development
Fertilization in Vitro*
Oocytes* / growth & development

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