Document Detail


In vitro fertility rate of 129 strain is improved by buserelin (gonadotropin-releasing hormone) administration prior to superovulation.
MedLine Citation:
PMID:  23097563     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 129 mice are well recognized for their low fertility and it is speculated that this lack of fertility may be due to the oocyte condition. In this study we investigated superovulation regimens for the 129S1/SvImJ mouse strain to improve the oocyte quality and fertility rate of in vitro fertilization (IVF). Female mice were divided into four groups based on hormone and timing of injection. Group 1 received pregnant mare serum gonadotropin (PMSG) and 48 h later human chorionic gonadotropin (hCG); using the same dose, group 2 received hCG 52 h post-PMSG and group 3, 55 h post-PMSG. Group 4 received buserelin (gonadotropin-releasing hormone agonist [GnRH]) followed 24 h later by PMSG and then hCG 55 h post-PMSG. IVF was performed using 129S1/SvImJ oocytes and sperm; C57BL/6J sperm with 129S1/SvImJ oocytes was used as fertility control. The IVF fertility rate was 1% (Groups 1 and 2), 17% (Group 3) and 55% (Group 4) for 129 oocytes fertilized with 129 sperm. For 129 oocytes fertilized with C57BL/6J sperm, the fertility rate was 5% (Group 1), 10% (Group 2), 40% (Group 3) and 59% (Group 4). These results suggest that extending the interval time between PMSG and hCG and giving GnRH in addition to the standard PMSG and hCG treatments can improve IVF fertility rate of 129S1/SvImJ mouse strains significantly.
Authors:
K Vasudevan; J M Sztein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Laboratory animals     Volume:  46     ISSN:  1758-1117     ISO Abbreviation:  Lab. Anim.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-25     Completed Date:  2013-03-25     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  0112725     Medline TA:  Lab Anim     Country:  England    
Other Details:
Languages:  eng     Pagination:  299-303     Citation Subset:  IM    
Affiliation:
Assisted Reproduction Technologies and Cryopreservation Unit, CMB, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Birth Rate
Buserelin / therapeutic use*
Chorionic Gonadotropin / pharmacology
Female
Fertility Agents, Female / therapeutic use*
Fertilization in Vitro / methods*
Gonadotropins, Equine / pharmacology
Mice*
Mice, 129 Strain
Oocytes / drug effects*
Superovulation / drug effects*
Time Factors
Grant Support
ID/Acronym/Agency:
Z99 AI999999/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Fertility Agents, Female; 0/Gonadotropins, Equine; 57982-77-1/Buserelin
Comments/Corrections

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