Document Detail


In vitro fermentative characteristics of ruminant diets supplemented with fibrolytic enzymes and ranges of optimal endo-beta-1,4-glucanase activity.
MedLine Citation:
PMID:  19364377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effectiveness of fibrolytic enzymes supplementing a range of forage to concentrate (F:C) diets was assessed with goat (G) or cow (C) inoculum using the gas production (GP) technique. Four F:C diets were evaluated: forage (1:0), high forage (0.7:0.3), medium forage (0.5:0.5) and low forage (0.3:0.7) diets, supplemented or not with Promote(TM) (PRO) at 1 or 2 ml/kg dry matter (DM). The GP kinetic was different between F:C (1:0 < 0.7:0.3 < 0.5:0.5 < 0.3:0.7) and inoculum. Responses to enzyme were positively related to forage level and differed with inoculum. The neutral detergent fibre and acid detergent fibre degradation were depressed by the concentrate in the substrates fermented with C and were not altered or even enhanced in G sets. Results confirm that increasing starch proportion modified the pattern of microbial fermentation, while no influences were detected in the improvement of cell wall degradation with fibrolytic enzymes. Another in vitro experiment was conducted to investigate factors by which endo-beta-1,4-glucanase activity (EA) of PRO is compromised in a factorial design (3 x 4 x 3) for three pH (4.0, 5.5 and 6.5), four temperatures (30, 40, 50 and 70 degrees C) and three doses (1, 2 and 3 ml/kg DM of substrate). Maximum EA were obtained for pH 4.0, 50 degrees C and 3 ml/kg DM. Optimal conditions for PRO proved to be outside the normal ranges in ruminal environment.
Authors:
E González-García; E Albanell; G Caja; R Casals
Related Documents :
8325797 - Effects of barley variety and restricted versus ad libitum intake on rate, site, and ex...
3676227 - Effects of supplements on intake, rumen function and nutrient supply and growth in catt...
16346277 - Occurrence of the rumen ciliate oligoisotricha bubali in domestic cattle (bos taurus).
10985417 - Effects of degree of fat saturation on fiber digestion and microbial protein synthesis ...
2231017 - Failure to demonstrate an effect of dietary fatty acid composition on body weight, body...
21983427 - Chronic subhepatotoxic exposure to arsenic enhances hepatic injury caused by high fat d...
Publication Detail:
Type:  Journal Article     Date:  2009-03-31
Journal Detail:
Title:  Journal of animal physiology and animal nutrition     Volume:  94     ISSN:  1439-0396     ISO Abbreviation:  J Anim Physiol Anim Nutr (Berl)     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-08-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101126979     Medline TA:  J Anim Physiol Anim Nutr (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  250-63     Citation Subset:  IM    
Affiliation:
Grup de Recerca en Remugants, Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis*
Animals
Bioreactors*
Cattle / physiology*
Diet / veterinary*
Enzymes / chemistry,  metabolism*
Female
Fermentation
Goats / physiology*
Hydrogen-Ion Concentration
Temperature
Chemical
Reg. No./Substance:
0/Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Supplementation of diets for lactating sows with zinc amino acid complex and gastric nutriment-intub...
Next Document:  Urinary calcium excretion in non-lactating dairy cows in relation to intake of fat-coated rice bran.