Document Detail


In vitro fermentation of a red wine extract by human gut microbiota: changes in microbial groups and formation of phenolic metabolites.
MedLine Citation:
PMID:  22313337     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An in vitro batch culture fermentation experiment was conducted with faecal inocula from three healthy volunteers in the presence and absence of a red wine extract. Changes in main bacterial groups were determined by FISH during a 48 h fermentation period. The catabolism of main flavonoids (i.e., flavan-3-ols and anthocyanins) and the formation of wide a range of phenolic microbial metabolites were determined by a targeted UPLC-PAD-ESI-TQ MS method. Statistical analysis revealed that catechol/pyrocatechol, as well as 4-hydroxy-5-(phenyl)-valeric, 3- and 4-hydroxyphenylacetic, phenylacetic, phenylpropionic, and benzoic acids showed the greatest increases observed during fermentation, whereas 5-(3'-hydroxyphenyl)--valerolactone, its open form 4-hydroxy-5-(3'-hydroxyphenyl)-valeric acid and 3,4-dihydroxyphenylacetic acid represented the largest inter-individual variations in the catabolism of red wine polyphenols. Despite these changes, microbial metabolism did not produce significant changes in the main bacterial groups detected although a slight inhibition of the Clostridium histolyticum group was observed.
Authors:
Fernando Sanchez-Patan; Carolina Cueva; Maria Monagas; Gemma E Walton; Glenn R Gibson; Rosa Lebrón-Aguilar; Jesus E Quintanilla; Pedro J Martin-Alvarez; M Victoria Moreno-Arribas; Begoña Bartolome
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-7
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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