Document Detail


In vitro expression of the restriction endonucleases LlaMI and ScrFI isolated from Lactococcus lactis M19 and UC503.
MedLine Citation:
PMID:  16144727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new restriction endonuclease LlaMI has been characterized in Lactococcus lactis subsp. cremoris M19. LlaMI recognizes the sequence 5'-CCNGG-3' and cuts after the second cytosine. This restriction endonuclease is related to commercially available ScrFI but not identical to it. Comparative analysis of the predicted amino acid sequences of LlaMI and ScrFI indicates five non-conservative amino acid changes between these two restriction enzymes. These two enzymes were expressed in vitro as histidine-tagged fusion proteins. LlaMI was shown to be more sensitive to high salt concentration than ScrFI. Southern blotting and hybridization analysis indicate that the gene for LlaMI R/M system is chromosomally encoded.
Authors:
George Szatmari; Ngiep Man Hua; Dimitri Vzdornov; François Daigle; Wanda Smoragiewicz; Margaret D Mamet-Bratley; Barbara Karska-Wysocki
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-06
Journal Detail:
Title:  Journal of biotechnology     Volume:  121     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-09     Completed Date:  2006-06-21     Revised Date:  2008-08-29    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  144-53     Citation Subset:  IM    
Affiliation:
Département de Microbiologie et Immunologie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Que., Canada H3C 3J7. george.szatmari@umontreal.ca
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Substitution*
Bacterial Proteins / biosynthesis,  genetics*,  isolation & purification
Deoxyribonucleases, Type II Site-Specific / biosynthesis,  genetics*,  isolation & purification
Gene Expression / genetics
Isoenzymes / biosynthesis,  genetics,  isolation & purification
Lactococcus lactis / enzymology,  genetics*
Sequence Analysis, Protein / methods
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Isoenzymes; EC 3.1.21.4/CCNGG-specific type II deoxyribonucleases; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific

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