| In vitro evidence of involvement of the epithelial y(+) transporter in β-defensin production on the ocular surface. | |
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MedLine Citation:
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PMID: 22056339 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To analyse the hypothesis as to whether there is a functional relationship between human cationic amino acid transporters (hCATs, y(+) transporter, the main transporter of l-arginine and l-lysine) and human β-defensin (important components of immune function) production on the ocular surface, arginase and nitrate monoxide synthase (NOS), enzymes that compete for l-arginine, were inhibited by norNOHA (N(omega)-hydroxy-nor-l-arginine) and/or L-NAME (NG-nitro-l-arginine methyl ester) in cultured human corneal epithelial cells. In addition, the transport activity of hCAT proteins was inhibited or activated through α-tocopherol or PMA (phorbol myristate acetate), respectively. Concentrations of the human inducible β-defensins (hBD) 2 and 3 were determined by ELISA experiments. The basic expression of hBD3 in non-stimulated HCE cells significantly exceeded that of hBD2. Both β-defensins also differed as to how readily their excretion could be stimulated. HBD2 excretion rate was 3.5 time more by L-NAME, whereas norNOHA had no effect. In contrast, hBD3 excretion was increased by norNOHA by a factor of 1.5 but L-NAME alone had no effect. The excretion of both β-defensins was increased 3- and 6-fold by combined administration of L-NAME, norNOHA and interleukin (IL)-1β. Administration of α-tocopherol increased hBD2 excretion twofold. No effect was observed for hBD3. With PMA, on the other hand, a reduction in secretion for both β-defensins was observed. These in vitro findings provide evidence of a functional association between CAT proteins and β-defensins 2 and 3 opening up a new field of research with pharmacological perspectives for treatment of inflammatory diseases such as keratitis or dry eye disease. |
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Authors:
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Kristin Jäger; Andrea Nielitz; Fabian Garreis; Saadettin Sel; Heike Nave; Friedrich P Paulsen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-14 |
Journal Detail:
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Title: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft Volume: - ISSN: 1618-0402 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100963897 Medline TA: Ann Anat Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier GmbH. |
Affiliation:
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Department of Anatomy and Cell Biology, Martin Luther University of Halle-Wittenberg, D-06097 Halle, Saale, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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