Document Detail

In vitro evaluation of the sinus sagittalis superior thrombosis model in the rat using 3D micro- and nanocomputed tomography.
MedLine Citation:
PMID:  19921163     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Thrombosis of the cerebral veins and sinus are common causes of stroke. Animal models help us to understand the underlying pathophysiology of this condition. Therefore, the purpose of our study was to evaluate a well-established model for sinus sagittalis (SSS) thrombosis using micro- and nanocomputed tomography (CT) imaging.
METHODS: SSS thrombosis was performed in four rats. After contrast perfusion, brains were isolated and scanned using micro-CT at (8 microm)(3) voxel size to generate 3D images of the cerebral vasculature. For more detailed information on vascular perfusion territories, nano-CT imaging was performed to investigate the boundary layer of contrast-enhanced vessels and the occluded veins. The venous and arterial vascular volume fraction and gray scale measurements were obtained in the SSS thrombosis group and compared to controls. The significance of differences in vascular volume fraction and gray scale measurements was tested with analysis of variance. Results were complemented with histology.
RESULTS: Micro-CT proved to accurately visualize and differentiate vascular occlusion territories performed in the SSS thrombosis model. Moreover, 3D micro-CT provided quantitative information on arterial and venous vascular volume fraction. Micro-CT imaging enables a total 3D visualization of complications (ventricle rupture) in the SSS thrombosis model. We established gray scale measurements by which focal cerebral ischemia could be radiographically categorized (p < 0.001).
CONCLUSIONS: Using nano-CT, the interface of contrast-perfused and occluded veins can be visualized. Micro-CT is feasible for analysis and differentiation of perfusion territories in an animal model of focal cerebral ischemia.
Alexander Claus Langheinrich; Mesut Yeniguen; Anne Ostendorf; Simone Marhoffer; Christian Dierkes; Susanne von Gerlach; Max Nedelmann; Marian Kampschulte; Georg Bachmann; Erwin Stolz; Tibo Gerriets
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-18
Journal Detail:
Title:  Neuroradiology     Volume:  52     ISSN:  1432-1920     ISO Abbreviation:  Neuroradiology     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-05     Completed Date:  2011-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302751     Medline TA:  Neuroradiology     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  815-21     Citation Subset:  IM    
Department of Radiology, Justus-Liebig University Giessen, Klinikstrasse 36, Giessen, Germany.
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MeSH Terms
Disease Models, Animal*
Imaging, Three-Dimensional / instrumentation,  veterinary*
Nanotechnology / instrumentation
Rats, Sprague-Dawley
Reproducibility of Results
Sagittal Sinus Thrombosis / radiography*
Sensitivity and Specificity
Superior Sagittal Sinus / radiography*
Tomography, X-Ray Computed / instrumentation,  veterinary*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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