Document Detail

In vitro evaluation of an epoxy resin's cytocompatibility using cell lines and human differentiated cells.
MedLine Citation:
PMID:  1863579     Owner:  NLM     Status:  MEDLINE    
The cytocompatibility of a polyepoxy resin (Elf Aquitaine) has been studied using both cell lines and human differentiated cell cultures. The human models were gingival fibroblasts and bone osteoblasts, while the cell lines were Hela cells and 3T3 Balb/c cells. Basal cytocompatibility was assessed by estimation of the cell proliferation, total cell protein content, cell membrane sub-lysis, and cell attachment and spreading. Specific cytocompatibility concerning human osteoblasts, from both alveolar and trabecular bone, was determined by measuring the intracellular alkaline phosphatase activity. Resin colonization by the cells was studied by both TEM and SEM. The behaviour of the two cell lines reveals a significant level of discrepancy, whereas the behaviour of human cells, whatever the model, is comparable; however, osteoblasts look more sensitive. Moreover, the results show that this epoxy resin exhibits a moderate cytocompatibility which could be the result of the cytotoxicity of early released products, associated with the considerable surface roughness.
M F Harmand; L Bordenave; R Bareille; A Naji; R Jeandot; F Rouais; D Ducassou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  2     ISSN:  0920-5063     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  1991  
Date Detail:
Created Date:  1991-09-11     Completed Date:  1991-09-11     Revised Date:  2008-02-20    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  67-79     Citation Subset:  IM    
INSERM-U. 306, Université de Bordeaux II, France.
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MeSH Terms
Alkaline Phosphatase / metabolism*
Biocompatible Materials*
Cell Adhesion / drug effects
Cell Differentiation / drug effects
Cell Division / drug effects
Cell Line
Chromium Radioisotopes / diagnostic use
Collagen / analysis
Epoxy Resins / pharmacology*
Osteoblasts / cytology,  drug effects*,  enzymology
Osteocalcin / analysis
Tumor Cells, Cultured
Reg. No./Substance:
0/Biocompatible Materials; 0/Chromium Radioisotopes; 0/Epoxy Resins; 104982-03-8/Osteocalcin; 9007-34-5/Collagen; EC Phosphatase

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