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In vitro evaluation of co-exposure of arsenium and an organic nanomaterial (fullerene, C(60)) in zebrafish hepatocytes.
MedLine Citation:
PMID:  21889614     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Taking into account the concept of the "Trojan Horse", where contaminants may have its entry into specific organs potentiated by its association with nanoparticles, the aim of this study was to analyze the joint toxic effects induced by an organic nanoparticle, fullerene (C(60)) with the metalloid arsenic (As(III)). Hepatocytes of zebrafish Danio rerio were exposed to As(III) (2.5 or 100μM), C(60) or As+C(60) for 4h, not altering cells viability. Intracellular reactive oxygen species concentration was reduced in cells exposed only to the C(60) (1mg/L) and in the treatment of 100μM As(III)+C(60). Co-exposure with C(60) abolished the peak of the antioxidant glutathione (GSH) registered in cells exposed to the lowest As(III) concentration (2.5μM). A similar result was observed in terms of lipid damage (TBARS). Total antioxidant capacity was significantly higher at both As(III) concentrations co-exposed to C(60) when compared with the control group. Activity of glutathione-S-transferase omega, a limiting enzyme in the methylation pathway of As(III), was reduced in the 100μM As(III)+C(60) treatment. Cells co-exposed to C(60) had a significantly higher accumulation of As(III), showing a "Trojan Horse" effect which did not result in higher cell toxicity. Instead, co-exposure of As(III) with C(60) showed to reduce cellular injury.
Authors:
Carmen L A Costa; Isabel S Chaves; Juliane Ventura-Lima; Josencler L Ribas Ferreira; Luis Ferraz; Leandro Machado de Carvalho; José M Monserrat
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-24
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  -     ISSN:  1532-0456     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-9-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Affiliation:
Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande-FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, ICB, FURG, Brazil.
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