| In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals. | |
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MedLine Citation:
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PMID: 21552710 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: The purpose of this study was to evaluate the effcacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. MATERIAL AND METHODS: Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups according to the auxiliary chemical substance used: G1) 2.5% sodium hypochlorite (NaOCl); G2) 2% chlorhexidine gel (CLX); G3) pyrogenfree solution. After, these groups were subdivided according to the intracanal medication (ICM): A) Calcium hydroxide paste (Calen®), B) polymyxin B, and C) Calcium hydroxide paste+2% CLX gel. For the control group (G4), pyrogen-free saline solution was used without application of intracanal medication. Samples of the root canal content were collected immediately after biomechanical preparation (BMP), at 7 days after BMP, after 14 days of intracanal medication activity, and 7 days after removal of intracanal medication. The following aspects were evaluated for all collections: a) antimicrobial activity; b) quantifcation of endotoxin by the Limulus Amebocyte Lysate test (LAL). Results were analyzed by the Kruskal-Wallis and Dunn's tests at 5% signifcance level. RESULTS: The 2.5% NaOCl and CLX were able to eliminate E. coli from root canal lumen and reduced the amount of endotoxin compared to saline. CONCLUSIONS: It was concluded that 2.5% NaOCl and CLX were effective in eliminating E. coli. Only the studied intracanal medications were to reduce the amount of endotoxin present in the root canals, regardless of the irrigant used. |
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Authors:
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Lilian Eiko Maekawa; Marcia Carneiro Valera; Luciane Dias de Oliveira; Cláudio Antonio Talge Carvalho; Cristiane Yumi Koga-Ito; Antonio Olavo Cardoso Jorge |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of applied oral science : revista FOB Volume: 19 ISSN: 1678-7765 ISO Abbreviation: J Appl Oral Sci Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-05-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101189774 Medline TA: J Appl Oral Sci Country: Brazil |
Other Details:
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Languages: eng Pagination: 106-12 Citation Subset: D; IM |
Affiliation:
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Department of Restorative Dentistry, São Paulo State University, São José dos Campos, SP, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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