Document Detail

In vitro establishment and characterization of a human megakaryoblastic cell line.
MedLine Citation:
PMID:  2310825     Owner:  NLM     Status:  MEDLINE    
A human megakaryoblastic cell line, designated CHRF-288-11, has been established in vitro through the use of adherent stromal cells in long-term human bone marrow culture. Long-term bone marrow cultures were required for the initial adaptation of the megakaryoblastic cells to culture conditions; however, once adapted, the cells were weaned from the stromal layer until they proliferated in the complete absence of any feeder layers. The seed cells for the establishment of this line were derived from a solid tumor; the cloned cell line derived from this tumor exhibits markers characteristic of megakaryocytes and platelets. Specifically, the cells express platelet peroxidase, platelet factor 4, and platelet Ca+(+)-adenosine triphosphatase (ATPase), glycoprotein IIb-IIIa (CDw41), factor VIII antigen, and the MY7 (CD13) and MY9 (CD33) antigens. The cells do not express the erythroid markers glycophorin A and hemoglobin, the myeloid marker myeloperoxidase, nor markers specific for T and/or B cells. The established cell line produces both basic fibroblast growth factor and transforming growth factor-beta, properties demonstrated previously for the solid tumor. The clonal cell population exhibited a unique, singular karyotype, indicating cellular homogeneity. The cells display a doubling time of approximately 33 hours in either 25% horse or calf serum. Treatment of the cells with 1 X 10(-8) mol/L phorbol 12-myristate 13-acetate (PMA) leads to the induction of multi-nucleation and hyperploidy in the cells, with approximately 35% of the cells exhibiting two or more nuclei per cell, and greater than 80% of the cells enlarging in size. The establishment of this unique cell line under defined culture conditions will be beneficial for the future study of megakaryocytic properties expressed by this cell line.
D A Fugman; D P Witte; C L Jones; B J Aronow; M A Lieberman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  75     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-17     Completed Date:  1990-04-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1252-61     Citation Subset:  AIM; IM    
Department of Hematology-Oncology, Children's Hospital Medical Center, Cincinnati, OH.
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MeSH Terms
Blotting, Northern
Bone Marrow / metabolism,  ultrastructure
Bone Marrow Cells
Cell Differentiation / drug effects
Cell Line
Cell Nucleus / drug effects,  ultrastructure
Cell Transformation, Neoplastic / drug effects,  pathology
Leukemia, Megakaryoblastic, Acute / pathology
Megakaryocytes / cytology*,  metabolism,  ultrastructure
Microscopy, Electron
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate

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