| In vitro effects of very low levels of aflatoxin B(1) on free radicals production and bactericidal activity of bovine blood neutrophils. | |
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MedLine Citation:
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PMID: 21377741 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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As one of the most potent and hazardous feed/food-originated mycotoxins, aflatoxin (AF) B(1) is regarded as a potent immunosuppressor in dairy cows. Neutrophils (PMN), as key effector cells against pathogens, have a high potential to kill engulfed microbes. To investigate the in vitro effects of very low doses of AFB(1) on blood PMN functions, we examined the effects of biologically relevant concentrations of AFB(1) on the phagocytosis and non-phagocytosis dependent luminol, representative of mainly intracellular free radicals, and isoluminol, representative of mainly extracellular free radicals, chemiluminescence (CL), necrosis and apoptosis of PMN. Isolated blood PMN from healthy dairy cows (n=12) were exposed to 0, 0.01, 0.05 and 0.5ng/ml of AFB(1) for 0.5 and 18h depending on the assay. Further, blood PMN of healthy dairy cows (n=8) were exposed to 0.5ng/ml of AFB(1) for 3h and myeloperoxidase (MPO) activity, superoxide anion (O(2)(-)) production, phagocytosis and killing activities against Staphylococcus (S.) aureus and Escherichia (E.) coli, were examined. Though the effect of extremely low doses of AFB(1) were less pronounced, at 0.5ng/ml the production of free radicals was greatly enhanced, especially extracellularly. In contrast to isoluminol CL, the AFB(1)-treated PMN showed a remarkably impaired phagocytosis-depended luminol CL. PMN necrosis and apoptosis were not affected by AFB(1). MPO activity, O(2)(-) production, phagocytosis rates and killing of E. coli and S. aureus by AFB(1)-treated PMN were significantly lower than those of non-treated ones. Our results show the extracellularly pro-oxidant and antiphagocytic properties of very low doses of AFB(1) for bovine PMN. The scope of the suppressive effects of the in vitro AFB(1) levels on cellular innate immune functions should be considered for high yielding dairy cows. |
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Authors:
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J Mehrzad; G Klein; J Kamphues; P Wolf; N Grabowski; H J Schuberth |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-1 |
Journal Detail:
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Title: Veterinary immunology and immunopathology Volume: - ISSN: 1873-2534 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-3-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8002006 Medline TA: Vet Immunol Immunopathol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Pathobiology, Faculty Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran; Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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