Document Detail

In vitro effects of pregnancy-associated plasma protein-A: artifacts due to heparin.
MedLine Citation:
PMID:  2460147     Owner:  NLM     Status:  MEDLINE    
Pregnancy-associated plasma protein-A (PAPP-A) has been reported to inhibit elastase activity, lymphoblastogenesis, complement activity, and thrombin-induced coagulation of fibrinogen. Since some of these results are controversial, we reevaluate here the effects of PAPP-A in these last two systems. By molecular sieve chromatography, PAPP-A immunoreactivity and inhibitory activity on thrombin and complement were dissociated. A PAPP-A-free washing of the heparin-Sepharose column used during the purification of PAPP-A showed inhibitory activities similar to those of purified PAPP-A. Furthermore, a preparation of PAPP-A that had not been submitted to heparin-Sepharose chromatography during purification was not active in either assays. Thus, the anticoagulant and anti-complement effects previously attributed to PAPP-A were due to a contaminant of low molecular mass. We believe that this contaminant is probably heparin. A protocol to eliminate free and PAPP-A-bound heparin is presented herein, and implications for other previously reported in vitro effects of PAPP-A are discussed.
A Meisser; A Geinoz; P Bischof
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  39     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-12-12     Completed Date:  1988-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  373-8     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University of Geneva, Switzerland.
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MeSH Terms
Blood Coagulation Tests
Complement Inactivator Proteins / physiology
Electrophoresis, Polyacrylamide Gel
Pregnancy Proteins / physiology*
Pregnancy-Associated Plasma Protein-A / isolation & purification,  physiology*
Reg. No./Substance:
0/Complement Inactivator Proteins; 0/Pregnancy Proteins; 9005-49-6/Heparin; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

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