| In vitro effects of pregnancy-associated plasma protein-A: artifacts due to heparin. | |
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MedLine Citation:
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PMID: 2460147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pregnancy-associated plasma protein-A (PAPP-A) has been reported to inhibit elastase activity, lymphoblastogenesis, complement activity, and thrombin-induced coagulation of fibrinogen. Since some of these results are controversial, we reevaluate here the effects of PAPP-A in these last two systems. By molecular sieve chromatography, PAPP-A immunoreactivity and inhibitory activity on thrombin and complement were dissociated. A PAPP-A-free washing of the heparin-Sepharose column used during the purification of PAPP-A showed inhibitory activities similar to those of purified PAPP-A. Furthermore, a preparation of PAPP-A that had not been submitted to heparin-Sepharose chromatography during purification was not active in either assays. Thus, the anticoagulant and anti-complement effects previously attributed to PAPP-A were due to a contaminant of low molecular mass. We believe that this contaminant is probably heparin. A protocol to eliminate free and PAPP-A-bound heparin is presented herein, and implications for other previously reported in vitro effects of PAPP-A are discussed. |
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Authors:
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A Meisser; A Geinoz; P Bischof |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of reproduction Volume: 39 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1988-12-12 Completed Date: 1988-12-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 373-8 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Geneva, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood Coagulation Tests Complement Inactivator Proteins / physiology Electrophoresis, Polyacrylamide Gel Hemolysis Heparin* Humans Pregnancy Proteins / physiology* Pregnancy-Associated Plasma Protein-A / isolation & purification, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Complement Inactivator Proteins; 0/Pregnancy Proteins; 9005-49-6/Heparin; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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