Document Detail


In vitro effects of melatonin on cell proliferation in a colon adenocarcinoma line.
MedLine Citation:
PMID:  10641013     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of melatonin on inhibition of cell growth was studied in CT-26, a murine colon carcinoma-derived cell line. Cells growing in exponential phase were exposed to low (10(-7)-10(-10) M) and high doses (1, 2 and 3 x 10(-3) M) of melatonin during 24 h. Synthesis of DNA was measured by 5-bromo-2'-deoxyuridine incorporation. There was no effect at low doses, but a statistically significant correlation was found between the decrease in DNA synthesis and the dose of melatonin used (r = -0.52, P < 0.001). This implied the following percentages of inhibition: 1 mM, 22%; 2 mM, 25%; 3 mM, 47%. Potential cell membrane damage by high doses of melatonin was investigated by lactate dehydrogenase measurement and no significant levels were observed. Analysis with a single saturation technique showed no detectable oestradiol receptors in this cell type; therefore, we can assume that the effects occurring with the addition of melatonin were not mediated by modulation of this hormone on oestrogen receptors. The decreases in cell growth were attributed to a moderate, but significant antiproliferative action of melatonin on this non-hormone-dependent cell line.
Authors:
M Farriol; Y Venereo; X Orta; J M Castellanos; T Segovia-Silvestre
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied toxicology : JAT     Volume:  20     ISSN:  0260-437X     ISO Abbreviation:  J Appl Toxicol     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2000-03-24     Completed Date:  2000-03-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8109495     Medline TA:  J Appl Toxicol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  21-4     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Affiliation:
Centro Investigaciones Metabólicas y Biologia Molecular (CIBBIM), Hospital General d'Hebron, Barcelona, Spain. farriol@hg.vhebron.es
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism,  pathology*
Animals
Cell Division / drug effects
Colonic Neoplasms / metabolism,  pathology*
DNA, Neoplasm / biosynthesis*
Dose-Response Relationship, Drug
L-Lactate Dehydrogenase / metabolism
Melatonin / pharmacology*
Mice
Receptors, Estradiol / metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Receptors, Estradiol; 73-31-4/Melatonin; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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