Document Detail

In vitro effects of folic acid on gamma-glutamyltransferase and glutathione reductase activities in malignant lung and thymus tumors.
MedLine Citation:
PMID:  11177297     Owner:  NLM     Status:  MEDLINE    
In vitro effects of folic acid (10(-5), 10(-4), and 10(-3)M) on activities of gamma-glutamyltransferase and glutathione reductase, the enzymes involved in glutathione metabolism, were studied in tissue samples obtained after surgical treatment of the lungs and thymus. Folic acid did not change gamma-glutamyltransferase activity in lung cancer tissue, but in thymoma tissue this substance in a concentration of 10(-3)M inhibited it by 16%. Folic acid had no effects on glutathione reductase activity in benign tumors and normal lung and thymus tissues, but increased this activity in thymoma and lung cancer tissues. Activation of glutathione reductase was probably related to binding of folic acid in the allosteric center of the enzyme, which probably induced conformational changes in the catalytic center, acceleration of electron transport from NADPH(2) to oxidized glutathione via flavin adenine nucleotide, and intense production of reduced glutathione.
A A Karelin; R N Korotkina; G N Matskevich; V V Borisov; A A Vishnevskii; L V Polikarpova; A G Kunitsyn
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Bulletin of experimental biology and medicine     Volume:  130     ISSN:  0007-4888     ISO Abbreviation:  Bull. Exp. Biol. Med.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372557     Medline TA:  Bull Exp Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  973-5     Citation Subset:  IM    
A. V. Vishnevskii Institute of Surgery, Russian Academy of Medical Sciences, Moscow.
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MeSH Terms
Dose-Response Relationship, Drug
Folic Acid / pharmacology*
Glutathione Reductase / drug effects*,  metabolism
Hamartoma / drug therapy,  enzymology
Lung Neoplasms / drug therapy,  enzymology*
Reference Values
Thymoma / drug therapy,  enzymology
Thymus Neoplasms / drug therapy,  enzymology*
Tuberculoma / drug therapy,  enzymology
gamma-Glutamyltransferase / drug effects*,  metabolism
Reg. No./Substance:
59-30-3/Folic Acid; EC Reductase; EC

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