| In vitro effects of bethanechol on specimens of intestinal smooth muscle obtained from the duodenum and jejunum of healthy dairy cows. | |
| | |
MedLine Citation:
|
PMID: 17331022 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To describe the in vitro effects of bethanechol on contractility of smooth muscle preparations from the small intestines of healthy cows and define the muscarinic receptor subtypes involved in mediating contraction. SAMPLE POPULATION: Tissue samples from the duodenum and jejunum collected immediately after slaughter of 40 healthy cows. PROCEDURES: Cumulative concentration-response curves were determined for the muscarinic receptor agonist bethanechol with or without prior incubation with subtype-specific receptor antagonists in an organ bath. Effects of bethanechol and antagonists and the influence of intestinal location on basal tone, maximal amplitude (A(max)), and area under the curve (AUC) were evaluated. RESULTS: Bethanechol induced a significant, concentration-dependent increase in all preparations and variables. The effect of bethanechol was more pronounced in jejunal than in duodenal samples and in circular than in longitudinal preparations. Significant inhibition of the effects of bethanechol was observed after prior incubation with muscarinic receptor subtype M(3) antagonists (more commonly for basal tone than for A(max) and AUC). The M(2) receptor antagonists partly inhibited the response to bethanechol, especially for basal tone. The M(3) receptor antagonists were generally more potent than the M(2) receptor antagonists. In a protection experiment, an M(3) receptor antagonist was less potent than when used in combination with an M(2) receptor antagonist. Receptor antagonists for M(1) and M(4) did not affect contractility variables. CONCLUSIONS AND CLINICAL RELEVANCE: Bethanechol acting on muscarinic receptor sub-types M(2) and M(3) may be of clinical use as a prokinetic drug for motility disorders of the duodenum and jejunum in dairy cows. |
| | |
Authors:
|
Julia B R Pfeiffer; Meike Mevissen; Adrian Steiner; Christopher J Portier; Mireille Meylan |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: American journal of veterinary research Volume: 68 ISSN: 0002-9645 ISO Abbreviation: Am. J. Vet. Res. Publication Date: 2007 Mar |
Date Detail:
|
Created Date: 2007-03-02 Completed Date: 2007-05-01 Revised Date: 2013-06-06 |
Medline Journal Info:
|
Nlm Unique ID: 0375011 Medline TA: Am J Vet Res Country: United States |
Other Details:
|
Languages: eng Pagination: 313-22 Citation Subset: IM |
Affiliation:
|
Clinic for Ruminants, Vetsuisse Faculty, University of Berne, 3012 Berne, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bethanechol / pharmacology* Cattle Dairying Dose-Response Relationship, Drug Duodenum* / drug effects Female Jejunum* / drug effects Muscle Contraction / drug effects* Muscle, Smooth / drug effects* Parasympatholytics / pharmacology Parasympathomimetics / pharmacology Piperidines / pharmacology Pirenzepine / analogs & derivatives, pharmacology Tropicamide / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
Z01 ES048002-20/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Parasympatholytics; 0/Parasympathomimetics; 0/Piperidines; 102394-31-0/otenzepad; 1508-75-4/Tropicamide; 28797-61-7/Pirenzepine; 674-38-4/Bethanechol; 81405-11-0/4-diphenylacetoxy-1,1-dimethylpiperidinium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro effects of reactive oxygen metabolites, with and without flunixin meglumine, on equine colo...
Next Document: Immunohistochemical evaluation of substance P and calcitonin gene-related peptide in skin and perios...