Document Detail


In vitro effects of bethanechol on specimens of intestinal smooth muscle obtained from the duodenum and jejunum of healthy dairy cows.
MedLine Citation:
PMID:  17331022     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To describe the in vitro effects of bethanechol on contractility of smooth muscle preparations from the small intestines of healthy cows and define the muscarinic receptor subtypes involved in mediating contraction.
SAMPLE POPULATION: Tissue samples from the duodenum and jejunum collected immediately after slaughter of 40 healthy cows.
PROCEDURES: Cumulative concentration-response curves were determined for the muscarinic receptor agonist bethanechol with or without prior incubation with subtype-specific receptor antagonists in an organ bath. Effects of bethanechol and antagonists and the influence of intestinal location on basal tone, maximal amplitude (A(max)), and area under the curve (AUC) were evaluated.
RESULTS: Bethanechol induced a significant, concentration-dependent increase in all preparations and variables. The effect of bethanechol was more pronounced in jejunal than in duodenal samples and in circular than in longitudinal preparations. Significant inhibition of the effects of bethanechol was observed after prior incubation with muscarinic receptor subtype M(3) antagonists (more commonly for basal tone than for A(max) and AUC). The M(2) receptor antagonists partly inhibited the response to bethanechol, especially for basal tone. The M(3) receptor antagonists were generally more potent than the M(2) receptor antagonists. In a protection experiment, an M(3) receptor antagonist was less potent than when used in combination with an M(2) receptor antagonist. Receptor antagonists for M(1) and M(4) did not affect contractility variables.
CONCLUSIONS AND CLINICAL RELEVANCE: Bethanechol acting on muscarinic receptor sub-types M(2) and M(3) may be of clinical use as a prokinetic drug for motility disorders of the duodenum and jejunum in dairy cows.
Authors:
Julia B R Pfeiffer; Meike Mevissen; Adrian Steiner; Christopher J Portier; Mireille Meylan
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of veterinary research     Volume:  68     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-02     Completed Date:  2007-05-01     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  313-22     Citation Subset:  IM    
Affiliation:
Clinic for Ruminants, Vetsuisse Faculty, University of Berne, 3012 Berne, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bethanechol / pharmacology*
Cattle
Dairying
Dose-Response Relationship, Drug
Duodenum* / drug effects
Female
Jejunum* / drug effects
Muscle Contraction / drug effects*
Muscle, Smooth / drug effects*
Parasympatholytics / pharmacology
Parasympathomimetics / pharmacology
Piperidines / pharmacology
Pirenzepine / analogs & derivatives,  pharmacology
Tropicamide / pharmacology
Grant Support
ID/Acronym/Agency:
Z01 ES048002-20/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Parasympatholytics; 0/Parasympathomimetics; 0/Piperidines; 102394-31-0/otenzepad; 1508-75-4/Tropicamide; 28797-61-7/Pirenzepine; 674-38-4/Bethanechol; 81405-11-0/4-diphenylacetoxy-1,1-dimethylpiperidinium
Comments/Corrections

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