| In vitro effect of sodium fluoride on antioxidative enzymes and apoptosis during murine odontogenesis. | |
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MedLine Citation:
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PMID: 20738751 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Excessive fluoride ingestion has been identified as a risk factor for fluorosis and oxidative stress. The oxidative stress results from the loss of equilibrium between oxidative and antioxidative mechanisms that can produce kinase activation, mitochondrial disturbance and DNA fragmentation, resulting in apoptosis. Actually many people are exposed to no-adverted fluoride consumption in acute or chronic way. The aim of this study was to determine the effect of sodium fluoride on first molar germ in relation to its effect on antioxidative enzymes immunoexpression and apoptosis. Thirty first molar germs from 1-day-old Balb/c mice were cultured for 24 h with sodium fluoride (0 mM, 1 mM and 5 mM). Immunoexpression determination of CuZnSod, MnSod, catalase, Bax, Bid, caspase 8, caspase 9, caspase 3 and TUNEL assay were performed. Cellular disorganization in ameloblast and odontoblast-papilla zones was observed. CuZnSod and MnSod immunoexpression decrease in experimental groups. Caspase 8, caspase 3, Bax, Bid increase expression and more TUNEL positive cells in both experimental groups than control, suggest that apoptosis induced by fluoride is related to oxidative stress due to reduction of the enzymatic antioxidant. |
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Authors:
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L F Jacinto-Alemán; J C Hernández-Guerrero; C Trejo-Solís; M D Jiménez-Farfán; A M Fernández-Presas |
Publication Detail:
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Type: Journal Article Date: 2010-08-03 |
Journal Detail:
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Title: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology Volume: 39 ISSN: 1600-0714 ISO Abbreviation: J. Oral Pathol. Med. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8911934 Medline TA: J Oral Pathol Med Country: Denmark |
Other Details:
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Languages: eng Pagination: 709-14 Citation Subset: D; IM |
Copyright Information:
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© 2010 John Wiley & Sons A/S. |
Affiliation:
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Laboratorio de Inmunología, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico. inmuno1@live.com.mx |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ameloblasts
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metabolism Animals Apoptosis* / physiology BH3 Interacting Domain Death Agonist Protein / biosynthesis Cariostatic Agents / toxicity* Caspases / biosynthesis Catalase / biosynthesis In Situ Nick-End Labeling Mice Mice, Inbred BALB C Odontogenesis / drug effects* Oxidative Stress* / physiology Reactive Oxygen Species / metabolism Sodium Fluoride / toxicity* Superoxide Dismutase / biosynthesis Tooth Germ / drug effects*, enzymology bcl-2-Associated X Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/BH3 Interacting Domain Death Agonist Protein; 0/Bid protein, mouse; 0/Cariostatic Agents; 0/Reactive Oxygen Species; 0/bcl-2-Associated X Protein; 7681-49-4/Sodium Fluoride; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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