Document Detail

In vitro differentiation of liver progenitor cells derived from healthy dog livers.
MedLine Citation:
PMID:  18454698     Owner:  NLM     Status:  MEDLINE    
Naturally occurring liver disease in dogs resemble human liver disease in great detail; including the activation of liver progenitor cells (LPC) in acute and chronic liver disease. The aim of the present study was to isolate, culture, and characterize progenitor cells derived from healthy mature dog livers. A nonparenchymal cell fraction enriched with small hepatocytes was isolated and cultured in Hepatozyme-serum-free media (SFM) to stimulate the growth of colony-forming small epithelial cells. After 2 weeks of culturing, clonal expansion of keratin 7 (K7) immunopositive small cells with a large nucleus/cytoplasm ratio emerged in the hepatocyte monolayer. These colonies expressed genes of several hepatocyte (CYP1A1, ALB, and KRT18), cholangiocyte/LPC (KRT7 and KRT19), and progenitor cell markers (alpha-fetoprotein, CD44, prominin1, KIT, THY1, and neural cell adhesion molecule 1), indicating their immature and bipotential nature. Gene-expression profiles indicated a more pronounced hepatic differentiation in Hepatozyme-SFM compared to William's Medium E (WME). Furthermore, colony-forming cells differentiated toward intermediate hepatocyte-like cells with a more pronounced membranous K7 immunostaining. In conclusion, colony-forming small epithelial cells in long-term canine liver cell cultures express LPC markers and have differentiating capacities. These cells may therefore be considered as progenitor cells of the liver.
Brigitte Arends; Bart Spee; Baukje A Schotanus; Tania Roskams; Ted S G A M van den Ingh; Louis C Penning; Jan Rothuizen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stem cells and development     Volume:  18     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-8     Citation Subset:  IM    
Department of Clinical Sciences of Companion Animals, Utrecht University, Utrecht, The Netherlands.
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MeSH Terms
Cell Differentiation*
Cell Separation
Cells, Cultured
Colony-Forming Units Assay
Gene Expression Profiling
Gene Expression Regulation
Keratin-7 / metabolism
Liver / cytology*,  metabolism
Stem Cells / cytology*,  metabolism
Reg. No./Substance:

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