Document Detail


In vitro differences between smooth muscle cells derived from varicose veins and normal veins.
MedLine Citation:
PMID:  19703751     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The theory of primary venous dilatation leading to secondary valvular incompetence and varicose vein formation has received more attention nowadays. Although many studies have investigated the role of the main components of the venous wall in the development of varicose veins, the leading cause remains unknown. The present study was designed to establish the role of smooth muscle cells (SMCs) of the tunica media on the pathogenesis of varicose veins by analyzing the phenotypic and functional differences between SMCs derived from varicose veins and normal veins.
METHODS: SMCs were isolated and cultured from saphenous veins of patients with varicose veins and normal veins. Cell proliferation and migration rates were compared. Expression of phenotype-dependent markers and matrix metalloproteinase-2 (MMP) production were analyzed by immunoblotting. Total collagen synthesis was evaluated by measuring the radioactivity of L-[3, 4-(3)H]proline in the media and the cell layer.
RESULTS: SMCs derived from varicose veins demonstrated increased proliferation (2-fold, P < .01), migration (3-fold, P < .001), MMP-2 production (3-fold, P < .01), and collagen synthesis (>2-fold, P < .001), with decreased expression of phenotype-dependent markers compared with SMCs derived from normal veins (P < .05).
CONCLUSION: SMCs derived from varicose veins are more dedifferentiated and demonstrate increased proliferative and synthetic capacity than SMCs derived from normal veins. These properties may contribute to the remodeling of the venous wall and the weakening of its antipressure capacity.
Authors:
Ying Xiao; Zhibin Huang; Henghui Yin; Ying Lin; Shenming Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-22
Journal Detail:
Title:  Journal of vascular surgery     Volume:  50     ISSN:  1097-6809     ISO Abbreviation:  J. Vasc. Surg.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-02     Completed Date:  2009-11-12     Revised Date:  2012-10-03    
Medline Journal Info:
Nlm Unique ID:  8407742     Medline TA:  J Vasc Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1149-54     Citation Subset:  IM    
Affiliation:
Research Center of Vascular Surgery, Department of Vascular Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Aged
Biological Markers / metabolism
Case-Control Studies
Cell Differentiation
Cell Movement
Cell Proliferation
Cells, Cultured
Collagen / metabolism
Female
Humans
Male
Matrix Metalloproteinase 2 / metabolism
Middle Aged
Muscle, Smooth, Vascular / metabolism,  pathology*
Myocytes, Smooth Muscle / metabolism,  pathology*
Phenotype
Saphenous Vein / metabolism,  pathology*
Time Factors
Tunica Media / metabolism,  pathology*
Varicose Veins / metabolism,  pathology*
Chemical
Reg. No./Substance:
0/Biological Markers; 9007-34-5/Collagen; EC 3.4.24.24/MMP2 protein, human; EC 3.4.24.24/Matrix Metalloproteinase 2

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