Document Detail

In vitro diagnosis of cow's milk protein sensitive enteropathy by organ culture method.
MedLine Citation:
PMID:  7227853     Owner:  NLM     Status:  MEDLINE    
The criteria that are used at present to diagnose cow's milk protein sensitive enteropathy (CMPSE) are based on an in vivo milk challenge which can be hazardous and life threatening. We have used an organ culture model to determine the usefulness of this technique in establishing the diagnosis of CMPSE on the basis of a single biopsy with in vitro milk challenge. Fourteen infants with diarrhoea clinically suspected to have CMPSE were studied prospectively. On the basis of milk challenge studies seven infants had CMPSE. They had clinical reaction to cow's milk with associated histological changes and depression of alkaline phosphatase levels in the jejunal mucosa. In all seven cases parallel changes in alkaline phosphatase levels were noted in the organ culture specimens of initial biopsy subjected to in vitro challenge. The seven control infants tolerated cow's milk and did not have histological changes. The alkaline phosphatase levels were moderately increased in the jejunal mucosa in five of the seven infants. The alkaline phosphatase levels in the organ culture specimens of initial biopsy were increased after in vitro challenge in all seven infants. This study suggests that organ culture methods may be useful in the vitro diagnosis of CMPSE, and also obviate the need for in vivo oral milk challenges and repeated biopsies.
N Iyngkaran; M Yadav; S Balabaskaran; E Sumithran
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gut     Volume:  22     ISSN:  0017-5749     ISO Abbreviation:  Gut     Publication Date:  1981 Mar 
Date Detail:
Created Date:  1981-07-23     Completed Date:  1981-07-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  199-202     Citation Subset:  AIM; IM    
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MeSH Terms
Alkaline Phosphatase / metabolism
Diarrhea, Infantile / diagnosis*,  etiology
Intestinal Mucosa / pathology
Jejunum / enzymology,  pathology
Malabsorption Syndromes / diagnosis*,  etiology
Milk Proteins / adverse effects*
Organ Culture Techniques
Reg. No./Substance:
0/Milk Proteins; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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