| In vitro deleterious effect of hypoxanthine in Ham's Nutrient Mixture F-10 culture medium on human oocyte fertilization and early embryonic development. | |
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MedLine Citation:
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PMID: 8224274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine whether hypoxanthine in Ham's Nutrient Mixture F-10 (GIBCO, Grand Island, NY) culture medium affects murine embryo development or the outcome of patients undergoing IVF-ET. DESIGN: For the two-cell embryo bioassay, embryos from each female were equally distributed and incubated in Ham's F-10 with or without hypoxanthine supplementation for up to 72 hours. To assess the effect of hypoxanthine in human IVF-ET, oocytes, sperm, and embryos were cultured in Ham's F-10 medium with or without hypoxanthine. Fertilization and embryo cleavage were assessed at 18 and 40 hours, respectively, after insemination. SETTING: University medical research laboratory. PATIENTS, PARTICIPANTS: Nine couples undergoing IVF-ET. RESULTS: Two-cell mouse embryos incubated for 24 hours without hypoxanthine developed 40% to morula, compared with 6.5% for the hypoxanthine group. At 72 hours, 99.5% of the embryos without hypoxanthine reached the expanded blastocyst stage with 65% of them exhibiting hatching, compared with 72% and 19.5%, respectively, for the group with hypoxanthine. Human oocytes cultured in Ham's F-10 without hypoxanthine showed higher fertilization rates than the group incubated in the presence of hypoxanthine (69% versus 53%). The proportion of cleaved embryos was not different between the two culture media; however, the rate of embryos cleaving without cytoplasmic fragments was significantly higher in the group without hypoxanthine (75% versus 35%). CONCLUSION: Ham's F-10 medium containing hypoxanthine significantly reduced embryo development in the two-cell mouse embryo bioassay. Hypoxanthine in culture medium for IVF-ET may have a deleterious effect on human gametes, leading to decreased fertilization and increased incidence of cytoplasmic fragments in the conceptuses. |
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Authors:
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M C Bastias; S T McGee-Belser; S H Bryan; J M Vasquez |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Fertility and sterility Volume: 60 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1993-12-01 Completed Date: 1993-12-01 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 876-80 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Blastocyst / drug effects* Culture Media Embryo Transfer* Embryonic and Fetal Development / drug effects* Female Fertilization in Vitro* Humans Hypoxanthine Hypoxanthines / toxicity* Isotonic Solutions* Male Mice Mice, Inbred Strains Morula / drug effects* Oocytes / drug effects, pathology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Hypoxanthines; 0/Isotonic Solutions; 0/nutrient mixture F-10 Ham; 68-94-0/Hypoxanthine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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