Document Detail


In vitro cytotoxicity of selected chemicals commonly produced during fire combustion using human cell lines.
MedLine Citation:
PMID:  15893446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fire combustion products contain a broad range of chemicals, which have a multitude of possible toxic interactions in humans. The aim of this study was to evaluate the cytotoxicity of selected substances commonly produced during fire combustion. A range of human cell lines and cultures including: skin fibroblasts, HepG2 (liver derived), and A549 (lung derived cells) were used to represent different human target organs. The colorimetric MTS assay (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) was used to detect the cytotoxic effects of selected substances including: acetic acid, ammonia, formaldehyde, hydrobromic acid, hydrochloric acid, hydrofluoric acid, potassium cyanide, sodium fluoride, sodium nitrite, sodium sulphide, and sulphurous acid. In this study, the NOAEC (No Observable Adverse Effect Concentration), IC(10) (10% inhibitory concentration), IC50 (50% inhibitory concentration), and TLC (Total Lethal Concentration) values were determined. The ratio between in vitro IC50 to in vivo human toxicity data (Lowest Lethal Dose-LDLo and Lowest Lethal Concentration--LCLo) was also established. Results indicated a strong relationship between IC50 values on the cell types used: fibroblast and A549 (R2: 0.92), A549 and HepG2 (R2: 0.72), fibroblast and HepG2 (R2: 0.69). Good correlation was obtained between the IC50 against LDLo and LCLo when an appropriate adjustment factor was implemented. Results of this study indicated that in vitro methods could be a potential technique for assessing the toxicity of fire combustion products.
Authors:
F Lestari; A J Hayes; A R Green; B Markovic
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  19     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-05-17     Completed Date:  2005-08-18     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  653-63     Citation Subset:  IM    
Affiliation:
Chemical Safety and Applied Toxicology (CSAT) Laboratories, School of Safety Science, The University of New South Wales, Sydney 2052, Australia. fatma@student.unsw.edu.au
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / toxicity
Ammonia / toxicity
Cell Line
Cell Survival / drug effects
Fires*
Formaldehyde / toxicity
Humans
Hydrobromic Acid / toxicity
Hydrofluoric Acid / toxicity
Potassium Cyanide / toxicity
Chemical
Reg. No./Substance:
10035-10-6/Hydrobromic Acid; 151-50-8/Potassium Cyanide; 50-00-0/Formaldehyde; 64-19-7/Acetic Acid; 7664-39-3/Hydrofluoric Acid; 7664-41-7/Ammonia

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