Document Detail


In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells.
MedLine Citation:
PMID:  22516759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: While waterpipe tobacco smoking has become a global phenomenon, its potential health consequences are poorly understood. In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD).
METHODS: The mainstream WSC was generated using a standard laboratory machine protocol. We assessed its mutagenicity using Ames test. In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation.
RESULTS: Within the range of tested doses, WSC did not elicit sufficient response to be considered mutagenic in any of the strains tested (TA98, TA100, TA102, and TA97a) but were found to be toxic for strains TA97a and TA102 at the highest tested doses. However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4.
CONCLUSION: The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation.
Authors:
Mayyasa Rammah; Farah Dandachi; Rola Salman; Alan Shihadeh; Marwan El-Sabban
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-04-10
Journal Detail:
Title:  Toxicology letters     Volume:  211     ISSN:  1879-3169     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-23     Completed Date:  2012-07-16     Revised Date:  2013-11-04    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  220-31     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 11072020, Lebanon.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Blotting, Western
Cell Aging / drug effects
Cell Cycle / drug effects
Cell Line, Tumor
Cell Nucleus / drug effects,  ultrastructure
Cell Proliferation / drug effects
Cell Survival / drug effects
Electric Impedance
Endothelial Cells / drug effects
Epithelial Cells / drug effects
Flow Cytometry
Fluorescent Antibody Technique
Humans
Matrix Metalloproteinases / biosynthesis
Mutagenicity Tests
Mutagens*
Nitric Oxide / analysis
Pulmonary Alveoli / cytology,  pathology*
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Smoke / adverse effects
Tobacco / toxicity*
Toll-Like Receptor 4 / biosynthesis
Grant Support
ID/Acronym/Agency:
R01 CA120142/CA/NCI NIH HHS; R01 DA025659/DA/NIDA NIH HHS; R01CA120142/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Mutagens; 0/Smoke; 0/Toll-Like Receptor 4; 10102-43-9/Nitric Oxide; EC 3.4.24.-/Matrix Metalloproteinases
Comments/Corrections

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