Document Detail

In vitro cytotoxicity evaluation of biomedical nanoparticles and their extracts.
MedLine Citation:
PMID:  19569209     Owner:  NLM     Status:  MEDLINE    
The present study presents a new approach for evaluating in vitro cytotoxicity of nanoparticles. The approach is based on American National Standard ISO 10993-5. Hepatoma HepG2 and fibroblast NIH3T3 cell lines were incubated with nanoparticles, and their associated extracts were derived at 70 and 121 degrees C. Nanoparticles proposed as potential biomedical imaging probes were evaluated on the basis of the detection of metabolic activities and cell-morphology changes. In general, nanoparticles incubated directly with cells showed higher cytotoxicity than their associated extracts. CdSe and core-shell CdSe@ZnS quantum dots resulted in low cell viability for both cell lines. The cytotoxicity of the quantum dots was attributed to the Cd ion and the presence of the nanoparticle itself. A statistically significant (p < 0.05) decrease in cell viability was found in higher dosage concentrations. Rare earth nanoparticles and their extracts appear to affect NIH3T3 cells only, with cell viability as low as 71.4% +/- 4.8%. Magnetic nanoparticles have no observable effects on the cell viabilities for both cell lines. In summary, we found the following: (1) both direct incubation and extracts of nanoparticles are required for complete assessment of nanoparticle cytotoxicity, (2) the rare earth oxide nanoparticles are less cytotoxic than the Cd-based quantum dots, and (3) the extent of cytotoxicity is dependent upon the cell line.
Gautom Kumar Das; Peggy P Y Chan; Ailing Teo; Joachim Say Chye Loo; James M Anderson; Timothy Thatt Yang Tan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  93     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-46     Citation Subset:  IM    
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
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MeSH Terms
Cadmium Compounds / toxicity
Cell Death / drug effects
Cell Extracts
Cell Shape / drug effects
Cell Survival / drug effects
Hep G2 Cells
NIH 3T3 Cells
Nanoparticles / toxicity*
Selenium Compounds / toxicity
Spectrum Analysis
Time Factors
Reg. No./Substance:
0/Cadmium Compounds; 0/Cell Extracts; 0/Selenium Compounds; 1306-24-7/cadmium selenide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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