Document Detail


In vitro cytotoxicity evaluation of biomedical nanoparticles and their extracts.
MedLine Citation:
PMID:  19569209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study presents a new approach for evaluating in vitro cytotoxicity of nanoparticles. The approach is based on American National Standard ISO 10993-5. Hepatoma HepG2 and fibroblast NIH3T3 cell lines were incubated with nanoparticles, and their associated extracts were derived at 70 and 121 degrees C. Nanoparticles proposed as potential biomedical imaging probes were evaluated on the basis of the detection of metabolic activities and cell-morphology changes. In general, nanoparticles incubated directly with cells showed higher cytotoxicity than their associated extracts. CdSe and core-shell CdSe@ZnS quantum dots resulted in low cell viability for both cell lines. The cytotoxicity of the quantum dots was attributed to the Cd ion and the presence of the nanoparticle itself. A statistically significant (p < 0.05) decrease in cell viability was found in higher dosage concentrations. Rare earth nanoparticles and their extracts appear to affect NIH3T3 cells only, with cell viability as low as 71.4% +/- 4.8%. Magnetic nanoparticles have no observable effects on the cell viabilities for both cell lines. In summary, we found the following: (1) both direct incubation and extracts of nanoparticles are required for complete assessment of nanoparticle cytotoxicity, (2) the rare earth oxide nanoparticles are less cytotoxic than the Cd-based quantum dots, and (3) the extent of cytotoxicity is dependent upon the cell line.
Authors:
Gautom Kumar Das; Peggy P Y Chan; Ailing Teo; Joachim Say Chye Loo; James M Anderson; Timothy Thatt Yang Tan
Related Documents :
20738069 - Auger-mediated cytotoxicity of cancer cells in culture by an 125i-antisense oligomer de...
19921589 - In vitro cytotoxic effects of senecio stabianus lacaita (asteraceae) on human cancer ce...
14711309 - Quantitative structure-activity relationships for gammadelta t cell activation by bisph...
16701969 - The combined use of human neural and liver cell lines and mouse hepatocytes improves th...
8308299 - An improved mtt assay using the electron-coupling agent menadione.
20063369 - Assessing nanotoxicity in cells in vitro.
23185989 - The mechanism of mtor (mammalian target of rapamycin) in a mouse model of polycystic ov...
6840209 - Establishment of nucleolar deficient sublines of ptk2 (potorous tridactylis) by ultravi...
16179749 - Effects of tomato paste extracts on cell proliferation, cell-cycle arrest and apoptosis...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  93     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-46     Citation Subset:  IM    
Affiliation:
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cadmium Compounds / toxicity
Cell Death / drug effects
Cell Extracts
Cell Shape / drug effects
Cell Survival / drug effects
Hep G2 Cells
Humans
Mice
NIH 3T3 Cells
Nanoparticles / toxicity*
Selenium Compounds / toxicity
Spectrum Analysis
Time Factors
Chemical
Reg. No./Substance:
0/Cadmium Compounds; 0/Cell Extracts; 0/Selenium Compounds; 1306-24-7/cadmium selenide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Solid lipid microparticles produced by spray congealing: Influence of the atomizer on microparticle ...
Next Document:  Citric acid-based elastomers provide a biocompatible interface for vascular grafts.