Document Detail


In vitro cytotoxicity of amorphous carbon films.
MedLine Citation:
PMID:  15623934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amorphous carbon (a-C), carbon nitride (a-CN) and titanium films were deposited on stainless steel substrates (SS) using a dc magnetron sputtering system attached to a high vacuum chamber. Films were deposited using a base pressure of 1.3x10(-4) Pa. For the carbon films a pure graphite target was eroded in an Argon plasma. For the case of the a-CN films, the Ar flux was substituted by 100% N2 gas. Titanium films were deposited in a different chamber, using a pure Ti target and an argon plasma. In vitro studies were carried out on the coated samples using human osteoblasts cells. Cytotoxicity of carbon films was assessed by cellular adhesion and proliferation, as determined by direct cellular counting using a spectroscopic technique and a well-defined standard curve. Osteoblasts cells were also grown on uncoated steel and prepared Petri dishes for comparison. The percentage of osteoblasts adhesion measured at 24 hrs attained maximum values for the a-C films. Similarly, cellular proliferation evaluated at three, five and seven days showed an outstanding increase of osteoblasts cells for the a-C and Ti coatings in contrast to the uncoated steel. The cell functionality was evaluated by the MTT test after incubation periods of 3, 5 and 7 days. The absorbance values obtained for a-C, a-CN and Ti surfaces resulted significantly higher with respect to the positive control, indicating that the surface did not induce any toxic effect. Preliminary bio-mineralization was evaluated by measuring the elemental composition of the mineral grown on the substrates after periods up to 14 days.
Authors:
S E Rodil; R Olivares; H Arzate
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bio-medical materials and engineering     Volume:  15     ISSN:  0959-2989     ISO Abbreviation:  Biomed Mater Eng     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-29     Completed Date:  2005-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9104021     Medline TA:  Biomed Mater Eng     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  101-12     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, Circuito exterior s/n, CU, 04510 México DF. ser38@zinalco.iimatercu.unam.mx
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MeSH Terms
Descriptor/Qualifier:
Bone Substitutes*
Carbon / chemistry*
Cell Adhesion / physiology
Cell Proliferation
Cells, Cultured
Coated Materials, Biocompatible / chemistry
Humans
Materials Testing
Osteoblasts / cytology*,  physiology*
Osteogenesis / physiology*
Titanium / chemistry*
Chemical
Reg. No./Substance:
0/Bone Substitutes; 0/Coated Materials, Biocompatible; 7440-32-6/Titanium; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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