Document Detail


In vitro cytokine release profile: predictive value for metastatic potential in head and neck squamous cell carcinomas.
MedLine Citation:
PMID:  23322448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Head and neck squamous cell carcinomas (HNSCCs) have devastating morbidity rates with mortality mainly because of metastasis.
METHODS: Multiplex enzyme-linked immunosorbent assay (ELISA) to assay a variety of cytokine levels secreted by a panel of stage-specific and anatomic site-specific primary, and recurrent and metastatic University of Michigan-HNSCC cell lines over a 72-hour time course.
RESULTS: Conditioned medium from metastatic or recurrent HNSCC showed significantly higher amounts of interleukin (IL)-6, IL-6 receptor, tumor growth factor-beta (TGF-β) and vascular endothelial growth factor (VEGF) than nonmetastatic cells or normal oral keratinocytes. Tumor necrosis factor (TNF) was only secreted by stage IV, metastatic, or recurrence-derived cell lines.
CONCLUSION: The cytokine profile of cultured HNSCC cells suggests that high levels of IL-6 and IL-6R, TGF-β, and VEGF are significantly related with their metastatogenic potential and provide rationale for determining if serum testing for a combination of these 4 soluble factors could be of predictive value for the HNSCC tumor progression and clinical outcome.
Authors:
Omar Shkeir; Maria Athanassiou-Papaefthymiou; Martian Lapadatescu; Petros Papagerakis; Michael J Czerwinski; Carol R Bradford; Thomas E Carey; Mark E P Prince; Gregory T Wolf; Silvana Papagerakis
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-16
Journal Detail:
Title:  Head & neck     Volume:  35     ISSN:  1097-0347     ISO Abbreviation:  Head Neck     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-10-23     Completed Date:  2014-07-14     Revised Date:  2014-09-09    
Medline Journal Info:
Nlm Unique ID:  8902541     Medline TA:  Head Neck     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1542-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / blood*,  secondary*
Cytokines / blood*
Enzyme-Linked Immunosorbent Assay
Head and Neck Neoplasms / blood*,  metabolism,  pathology,  secondary
Humans
Interleukin-6 / blood
Predictive Value of Tests
Prognosis
Reference Values
Statistics, Nonparametric
Transforming Growth Factor beta1 / blood
Tumor Cells, Cultured / cytology,  metabolism
Vascular Endothelial Growth Factor A / blood
Grant Support
ID/Acronym/Agency:
P30 CA046592/CA/NCI NIH HHS; P30 CA46592/CA/NCI NIH HHS; P50 CA 97248/CA/NCI NIH HHS; P50 CA097248/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-6; 0/Transforming Growth Factor beta1; 0/Vascular Endothelial Growth Factor A
Comments/Corrections

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