| In vitro culture of intramolluscan stages of the avian schistosome Trichobilharzia ocellata. | |
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MedLine Citation:
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PMID: 3892958 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several different procedures for in vitro cultivation of intramolluscan stages of the avian schistosome Trichobilharzia ocellata were tried. A medium was found and culture conditions were established that not only supported in vitro transformation of miracidia into mother sporocysts, but also resulted in substantial subsequent growth; moreover, some degree of germinative development appeared to occur as well. Cerebral ganglia from uninfected adult snails of the intermediate host species, Lymnaea stagnalis, could produce factors promoting in vitro development of young mother sporocysts. Results are compared with data from the literature and it is concluded that greater success in in vitro culturing of young mother sporocysts of T. ocellata can be achieved than has hitherto been reported for other schistosome species. The same culture procedures were less successful when applied to other intramolluscan stages of T. ocellata, but can be used for in vitro maintenance of these stages. The procedures described here will be a useful tool in the study of schistosome-snail interactions in T. ocellata-L. stagnalis and possibly in other systems as well. |
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Authors:
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J J Mellink; W van den Bovenkamp |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Zeitschrift für Parasitenkunde (Berlin, Germany) Volume: 71 ISSN: 0044-3255 ISO Abbreviation: Z Parasitenkd Publication Date: 1985 |
Date Detail:
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Created Date: 1985-08-12 Completed Date: 1985-08-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8710749 Medline TA: Z Parasitenkd Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 337-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Culture Media Female Hemolymph / parasitology Host-Parasite Interactions Lymnaea / parasitology* Microscopy, Phase-Contrast Schistosomatidae / anatomy & histology, growth & development* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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