Document Detail


In vitro culture of equine respiratory mucosa explants.
MedLine Citation:
PMID:  18539059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An in vitro model of the upper respiratory tract of the horse was developed to investigate mechanisms of respiratory diseases. Four tissues of the upper respiratory tract of three horses were collected. Explants were maintained in culture at an air-liquid interface for 96h. At 0, 24, 48, 72 and 96h of cultivation, a morphometric analysis was performed using light microscopy, scanning electron microscopy and transmission electron microscopy. The explants were judged on morphometric changes of epithelium, basement membrane and connective tissue. Viability was evaluated using a fluorescent Terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labelling (TUNEL) staining. No significant changes in morphometry and viability of any of the explants were observed during cultivation. Hence, the in vitro model may be useful to study infectious and non-infectious diseases at the level of the equine respiratory tract, with potential application to the development of vaccines and treatments for diseases of the respiratory tract.
Authors:
Annelies Vandekerckhove; Sarah Glorieux; Wim Van den Broeck; Annick Gryspeerdt; Karen M van der Meulen; Hans J Nauwynck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-06
Journal Detail:
Title:  Veterinary journal (London, England : 1997)     Volume:  181     ISSN:  1090-0233     ISO Abbreviation:  Vet. J.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706281     Medline TA:  Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  280-7     Citation Subset:  IM    
Affiliation:
Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cilia
Horses / physiology*
Microscopy
Nasopharynx
Palatine Tonsil / anatomy & histology*,  physiology*
Respiratory Mucosa / anatomy & histology*,  physiology*
Time Factors
Tissue Culture Techniques / veterinary*
Trachea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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