Document Detail


In vitro culture of cells isolate from dimethylnitrosamine-induced renal mesenchymal tumors of the rat. I. Qualitative morphology.
MedLine Citation:
PMID:  1127737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The in vitro morphology of cells isolated from renal mesenchymal tumors induced in rats by a single ip injection of dimethylnitrosamine was followed in short- and long-term culture. The morphology of cells isolated from normal rat-kidney cortex was studied for comparison. Primary tumor-cell isolates consisted of a range of mesenchymal cell forms interspersed between discrete, homogeneous islands of cohesive epithelium-like cells. The latter were presumably derived from renal parenchyma that had been engulfed within the in vivo neoplasm by proliferating tumor cells, since this epithelial component did not survive serial subculture. Established tumor cell lines consisted of a pleomorphic range of mesenchymal forms resembling the descriptions of various cell types in vitro, including fibroblasts, smooth muscle cells, and endothelial cells. A range of mesenchymal cell forms in culture therefore correlated with the heterogeneous histologic nature of the in vivo neoplasm in which fibroblasts, smooth muscle fibers, and endothelium-like cells were also represented. Multinucleate or polymorphonuclear giant cells were characteristic of tumor cell cultures, whereas large, expanded polygonal cells with longitudinal striations were characteristic of normal kidney cell cultures. Some tumor cell cultures were typified by cells with cytoplasmic vacuolation and some by the acquisition of an epithelioid form at high cell density, a change not preceded by islands of cohesive, epithelium-like cells at preconfluent stages.
Authors:
G C Hard; R Borland
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of the National Cancer Institute     Volume:  54     ISSN:  0027-8874     ISO Abbreviation:  J. Natl. Cancer Inst.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-08-08     Completed Date:  1975-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503089     Medline TA:  J Natl Cancer Inst     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1085-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / ultrastructure
Cells, Cultured
Dimethylnitrosamine*
Female
Kidney Cortex / cytology
Kidney Neoplasms / chemically induced,  pathology*
Male
Neoplasms, Experimental / chemically induced,  pathology
Nitrosamines*
Rats
Time Factors
Vacuoles / ultrastructure
Chemical
Reg. No./Substance:
0/Nitrosamines; 62-75-9/Dimethylnitrosamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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