Document Detail

In vitro cultivation of Anaplasma marginale and A. phagocytophilum in tick cell lines: a review.
MedLine Citation:
PMID:  22832744     Owner:  NLM     Status:  MEDLINE    
Continuous cell lines have been established from several ixodid and argasid tick species, representing an excellent tool suitable for the isolation of pathogens and their subsequent propagation, which in turn allows the production of antigenic material for diagnostic tests, antibody and vaccine production, and also for studies on host-vector-pathogen relationships. This paper reviews the use of tick cells for culture initiation and maintenance of two obligate intracellular bacterial pathogens, Anaplasma marginale and Anaplasma phagocytophilum. These in vitro cultivation systems have been used in a wide range of studies, covering morphological ultrastructural analysis, genetics, proteomics and biological differences between strains, including genome transcriptional and protein expression approaches, enabling comparisons between host and vector cells. Thus, such systems open a new window for a better understanding of interactions between pathogens and tick cells. Last but not least, such systems contribute to the reduction in usage of animals for experimental research, as antigenic material can be produced in reasonably large quantities without the use of in vivo species-specific systems.
Lygia Maria Friche Passos
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária     Volume:  21     ISSN:  1984-2961     ISO Abbreviation:  Rev Bras Parasitol Vet     Publication Date:    2012 Apr-Jun
Date Detail:
Created Date:  2012-07-26     Completed Date:  2013-01-22     Revised Date:  2013-08-13    
Medline Journal Info:
Nlm Unique ID:  9440482     Medline TA:  Rev Bras Parasitol Vet     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  81-6     Citation Subset:  IM    
Departamento de Medicina Veterinária Preventiva, Instituto Nacional em Ciência e Tecnologia--Informação Genético-Sanitária da Pecuária Brasileira, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
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MeSH Terms
Anaplasma marginale / growth & development*
Anaplasma phagocytophilum / growth & development*
Bacteriological Techniques / methods
Cell Line
Ticks / cytology

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