| In vitro cow's milk protein-specific inflammatory and regulatory cytokine responses in preterm infants with necrotizing enterocolitis and sepsis. | |
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MedLine Citation:
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PMID: 20975616 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Enteral feeding with cow's milk formula is associated with neonatal necrotizing enterocolitis (NEC) and sepsis. Dietary antigen sensitization may play a role in promoting and/or sustaining inflammation in both conditions. Aiming at investigating cow's milk protein (CMP)-specific cytokine responses in preterm infants with NEC and sepsis, 14 babies with NEC, 14 matched healthy controls, and 10 septic controls were recruited. Unstimulated and stimulated peripheral blood mononuclear cells (PBMCs) secreting IFN-γ, IL-4, IL-10, and TGF-β1 were counted by the single-cell enzyme-linked immunospot (ELISPOT) assay. During the acute phase of NEC, patients showed a general pattern of a high level of cytokine secretion both when unstimulated and stimulated by mitogen [phytohaemagglutinin (PHA)] and CMPs: beta-lactoglobulin (β-lg) and casein. These responses were more marked to β-lg for IFN-γ, IL-4, and IL-10 than TGF-β1. Cytokine responses in sepsis were lower than in NEC (lowest in healthy controls, with a minimal TGF-β1 response). At term, lower frequencies of cytokine-secreting cells were elicited than during the acute phase, except for TGF-β1 secreting cells, which increased at term (in response to PHA and CMPs) particularly following not only NEC but also sepsis. |
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Authors:
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Adel E Abdelhamid; Shu-Ling Chuang; Peter Hayes; John M E Fell |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pediatric research Volume: 69 ISSN: 1530-0447 ISO Abbreviation: Pediatr. Res. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
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Languages: eng Pagination: 165-9 Citation Subset: IM |
Affiliation:
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Division of Paediatrics, Imperial College, London SW10 9NH, United Kingdom. adelabdelhamid@yahoo.co.uk |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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