Document Detail

In vitro correction of the interleukin-2 and interferon-gamma defect in multiple sclerosis.
MedLine Citation:
PMID:  3935348     Owner:  NLM     Status:  MEDLINE    
The effect of phytohaemagglutinin (PHA) and/or phorbol myristic acetate (PMA) on human interferon-gamma (HuIFN-gamma) and interleukin 2 (IL-2) production was measured in peripheral blood leucocytes (PBL) from multiple sclerosis (MS) patients. Seven out of 12 MS patients studied had PBLs which were unable to produce any detectable HuIFN-gamma after stimulation with PHA. The PBL cultures of the same seven patients were also defective for IL-2 production after PHA stimulation. Addition of PMA before and during PHA stimulation resulted in restored IL-2 and HuIFN-gamma production in all otherwise non-responsive cultures. In MS cultures responsive to PHA alone, the addition of PMA resulted in a 10-fold increase of HuIFN-gamma and IL-2 production. Analysis of interleukin 1 (IL-1) production by peripheral blood monocytes (PBM) isolated from the same cultures, revealed that both spontaneous and PMA-induced IL-1 yield was the same for all cultures tested, regardless whether they produced IL-2 and HuIFN-gamma after PHA stimulation or not.
G Vervliet; L Schandené
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  61     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1986-01-30     Completed Date:  1986-01-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  556-61     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Interferon-gamma / biosynthesis*
Interleukin-1 / biosynthesis
Interleukin-2 / biosynthesis*
Leukocytes / metabolism
Lymphocyte Activation
Monocytes / metabolism
Multiple Sclerosis / immunology*
Phytohemagglutinins / pharmacology
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Interleukin-1; 0/Interleukin-2; 0/Phytohemagglutinins; 16561-29-8/Tetradecanoylphorbol Acetate; 82115-62-6/Interferon-gamma

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