Document Detail

In vitro comparison of equine granulocytes labeled with 99mTc-hexamethylpropyleneamine oxime or 111In-oxine.
MedLine Citation:
PMID:  1626776     Owner:  NLM     Status:  MEDLINE    
Isolated equine granulocytes (WBC), radiolabeled with 99mTc-hexamethylpropyleneamine oxime (99mTc-HMPAO) or 111In-oxine, were evaluated in vitro for their labeling characteristics, viability, and phagocytic function over a 6-hour postlabeling period. Mean +/- SD labeling efficiency for 111In-oxine-WBC was 62.2 +/- 15.3%, which was significantly (P less than 0.001) higher than that for 99mTc-HMPAO-WBC (32.0 +/- 17.0%). In vitro elution of radiolabel from cells was significantly (P less than 0.02) greater for 99mTc-HMPAO-WBC at 0.5, 2, and 4 hours, but was not significantly different from elution of radiolabel for 111In-oxine-WBC at 6 hours. Viability, assessed by trypan blue dye exclusion, for 99mTc-HMPAO-WBC, 111In-oxine-WBC, and nonlabeled control WBC ranged from 97 to 100%, and was not significantly different among groups. Cell function was assessed by use of a phagocytosis assay and was reported as phagocytic index. The phagocytic index ranged from 0.86 to 0.96 for 99mTc-HMPAO-WBC, and from 0.76 to 0.97 for 111In-oxine-WBC. The phagocytic index was not significantly different at 0.5, 2, or 4 hours, but was significantly (P = 0.038) greater at 6 hours for 99mTc-HMPAO-WBC. Because of the superior imaging characteristics of 99mTc-HMPAO-WBC and equal or better labeling characteristics than those for 111In-oxine at 6 hours, 99mTc-HMPAO-WBC appear to be a good alternative to 111In-oxine-WBC.
G B Daniel; R L Tucker; T Buckman; S L Daniel
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of veterinary research     Volume:  53     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-08-07     Completed Date:  1992-08-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  871-6     Citation Subset:  IM    
Department of Urban, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.
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MeSH Terms
Cell Survival
Cells, Cultured
Granulocytes / cytology*,  immunology
Horses / blood*
Isotope Labeling / veterinary
Leukocyte Count / veterinary
Organometallic Compounds*
Organotechnetium Compounds*
Oxyquinoline / analogs & derivatives*
Technetium Tc 99m Exametazime
Reg. No./Substance:
0/Organometallic Compounds; 0/Organotechnetium Compounds; 0/Oximes; 100504-35-6/Technetium Tc 99m Exametazime; 14514-42-2/indium oxine; 148-24-3/Oxyquinoline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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