Document Detail


In vitro chemotactic response of osteoblast-like osteosarcoma cells to a partially purified protein extract of demineralized bone matrix.
MedLine Citation:
PMID:  2002426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this investigation was to evaluate the chemotactic potential of a partially purified protein extract from bone matrix when tested against osteosarcoma cells with osteoblast characteristics. The chemotactic response of ROS 17/2 cells to a lyophilized bovine bone extract purified to "Urist step eight" was evaluated in Boyden blind well chambers. A checkerboard design was employed to test cell migration against positive, negative, and no concentration gradients, thereby controlling the effects of chemokinesis and/or random migration on results. The results demonstrate that the partially purified protein extract from bovine bone matrix is chemotactic since more cells migrated to positive gradients than to negative gradients (P less than .01). The chemotactic effect was confirmed by an increase in cell migration toward positive gradients of the bone extract compared to cell migration in the presence of no gradient (P less than .01). When no gradient was present, the cells exhibited an increased response in the presence of equal concentrations of the bone extract (P less than .01) indicating a chemokinetic effect. The proteinaceous nature of the chemoattractant was confirmed by its susceptibility to trypsin digestion and heat exposure.
Authors:
R A Padley; C M Cobb; W J Killoy; N L Newhouse; B D Boyan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of periodontology     Volume:  62     ISSN:  0022-3492     ISO Abbreviation:  J. Periodontol.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-04-12     Completed Date:  1991-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15-20     Citation Subset:  D; IM; S    
Affiliation:
School of Dentistry, Department of Periodontics, University of Missouri-Kansas City.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Matrix
Bone Morphogenetic Proteins
Cattle
Cell Movement
Cells, Cultured
Chemotaxis* / drug effects
Growth Substances / pharmacology*
Neoplasm Proteins / pharmacology*
Osteoblasts / physiology*
Osteosarcoma
Proteins / pharmacology*
Rats
Chemical
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/Growth Substances; 0/Neoplasm Proteins; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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