Document Detail


In vitro chemosensitivity of two Ewing's sarcoma cell lines: implication for autologous bone marrow transplantation.
MedLine Citation:
PMID:  2620246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intensive chemotherapy requiring rescue with autologous bone marrow may be an encouraging mode of treatment for poor prognosis Ewing's sarcoma (ES). In vitro chemosensitivity test may be useful for establishing an effective purging condition. We studied the in vitro effects of a variety of chemotherapeutic agents on two established ES cell lines (ES-5838 and ES-A4573) and marrow colony-forming unit-granulocyte macrophage (CFU-GM). 4-Hydroperoxycyclophosphamide (4-HC), at 100 microM produced complete inhibition (greater than 5 log) of clonogenic growth of both ES cell lines and spared 6.9% of normal CFU-GM growth. Etoposide (VP-16), at 100 microM produced 3-3.5 log inhibition of ES cell lines and complete inhibition of CFU-GM growth. Adriamycin (ADR) and vincristine (VCR) were more cytotoxic to ES-5838 cells than ES-A4573 cells. ADR at 1 microM produced 99.7% inhibition of ES-5838 cells, 92.2% of ES-A4573 cells, and 86% inhibition of CFU-GM. VCR at 1 microM produced 98.6% inhibition of ES-5838 cells, only 43.7% of ES-A4573 cells, and 75% inhibition of CFU-GM growth. Addition of verapamil did not enhance VCR cytotoxicity of ES cell lines. These studies indicate that 4-HC may be a useful agent for purging metastatic ES cells from the bone marrow for autologous marrow transplantation.
Authors:
S C Gulati; J H Kwon; J Atzpodien; T J Triche; O M Colvin; B D Clarkson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer investigation     Volume:  7     ISSN:  0735-7907     ISO Abbreviation:  Cancer Invest.     Publication Date:  1989  
Date Detail:
Created Date:  1990-03-20     Completed Date:  1990-03-20     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8307154     Medline TA:  Cancer Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  411-6     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York, New York.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Bone Marrow / drug effects
Bone Marrow Transplantation*
Cyclophosphamide / analogs & derivatives,  pharmacology
Hematopoietic Stem Cells / drug effects
Humans
Sarcoma, Ewing's / pathology*,  therapy
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 39800-16-3/perfosfamide; 50-18-0/Cyclophosphamide

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