Document Detail


In vitro cell culture models to study the corneal drug absorption.
MedLine Citation:
PMID:  21381983     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Many diseases of the anterior eye segment are treated using topically applied ophthalmic drugs. For these drugs, the cornea is the main barrier to reaching the interior of the eye. In vitro studies regarding transcorneal drug absorption are commonly performed using excised corneas from experimental animals. Due to several disadvantages and limitations of these animal experiments, establishing corneal cell culture models has been attempted as an alternative.
AREAS COVERED: This review summarizes the development of in vitro models based on corneal cell cultures for permeation studies during the last 20 years, starting with simple epithelial models and moving toward complex organotypical 3D corneal equivalents.
EXPERT OPINION: Current human 3D corneal cell culture models have the potential to replace excised animal corneas in drug absorption studies. However, for widespread use, the contemporary validation of existent systems is required.
Authors:
Stephan Reichl; Christian Kölln; Matthias Hahne; Jessica Verstraelen
Related Documents :
21235283 - Mechanistic enhancement of the intestinal absorption of drugs containing the polar guan...
21446053 - Induction of drug efflux protein expression by venlafaxine but not desvenlafaxine.
12711173 - The effect of formulation variables on the stability of nebulized aviscumine.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2011-03-08
Journal Detail:
Title:  Expert opinion on drug metabolism & toxicology     Volume:  7     ISSN:  1744-7607     ISO Abbreviation:  Expert Opin Drug Metab Toxicol     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-19     Completed Date:  2011-08-04     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  101228422     Medline TA:  Expert Opin Drug Metab Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  559-78     Citation Subset:  IM    
Affiliation:
Technische Universita¨t Braunschweig, Institut fu¨r Pharmazeutische Technologie, Braunschweig, Germany. S.Reichl@tu-bs.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animal Testing Alternatives
Animals
Cell Culture Techniques
Cornea / metabolism*
Epithelium, Corneal / metabolism
Eye Diseases / drug therapy
Humans
Models, Biological*
Permeability
Pharmaceutical Preparations / administration & dosage*,  metabolism
Chemical
Reg. No./Substance:
0/Pharmaceutical Preparations

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Epigenetic regulation in myelodysplastic syndromes: implications for therapy.
Next Document:  A phase I/IIa clinical trial of a recombinant Rho protein antagonist in acute spinal cord injury.