Document Detail

In vitro cell culture models to study the corneal drug absorption.
MedLine Citation:
PMID:  21381983     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Many diseases of the anterior eye segment are treated using topically applied ophthalmic drugs. For these drugs, the cornea is the main barrier to reaching the interior of the eye. In vitro studies regarding transcorneal drug absorption are commonly performed using excised corneas from experimental animals. Due to several disadvantages and limitations of these animal experiments, establishing corneal cell culture models has been attempted as an alternative.
AREAS COVERED: This review summarizes the development of in vitro models based on corneal cell cultures for permeation studies during the last 20 years, starting with simple epithelial models and moving toward complex organotypical 3D corneal equivalents.
EXPERT OPINION: Current human 3D corneal cell culture models have the potential to replace excised animal corneas in drug absorption studies. However, for widespread use, the contemporary validation of existent systems is required.
Stephan Reichl; Christian Kölln; Matthias Hahne; Jessica Verstraelen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2011-03-08
Journal Detail:
Title:  Expert opinion on drug metabolism & toxicology     Volume:  7     ISSN:  1744-7607     ISO Abbreviation:  Expert Opin Drug Metab Toxicol     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-19     Completed Date:  2011-08-04     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  101228422     Medline TA:  Expert Opin Drug Metab Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  559-78     Citation Subset:  IM    
Technische Universita¨t Braunschweig, Institut fu¨r Pharmazeutische Technologie, Braunschweig, Germany.
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MeSH Terms
Administration, Topical
Animal Testing Alternatives
Cell Culture Techniques
Cornea / metabolism*
Epithelium, Corneal / metabolism
Eye Diseases / drug therapy
Models, Biological*
Pharmaceutical Preparations / administration & dosage*,  metabolism
Reg. No./Substance:
0/Pharmaceutical Preparations

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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