Document Detail

In vitro biodegradation of polyhydroxybutyrate-hydroxyvalerate microcapsules exposed to Hank's buffer, newborn calf serum, pancreatin and synthetic gastric juice.
MedLine Citation:
PMID:  8994074     Owner:  NLM     Status:  MEDLINE    
Spherical microporous reservoir-type microcapsules, fabricated using a W/O/W double emulsion technique with solvent evaporation and composed of 330 kD poly(beta-hydroxybutyrate-hydroxyvalerate (P(HB-HV)) (10.8% HV)/20% PCL II containing a range of bovine serum albumin (BSA) loadings, were incubated in Hank's buffer, pH 7.4, newborn calf serum (NCS), 1.5% pancreatin and synthetic gastric juice containing 10% pepsin over 30 days, and their percentage weight loss (PWL) and change in ultrastructural morphology monitored by gravimetry and stereoscan electron microscopy (SEM), respectively. The greatest percentage weight loss from microcapsules was observed after incubation in NCS and decreased in the other NCS > pancreatin > synthetic gastric juice > Hank's buffer. Only 5, 10 and 15% bovine serum albumin (BSA) loaded microcapsules incubated in Hank's buffer and synthetic gastric juice showed a significant increase in PWL with increasing percentage BSA loading. The overall sequence of changes in structural morphology due to biodegradation occurred at different rates in the different 'physiological' media. An initial increase in micropore diameter was followed by the coalescence of microspores to form macroporous pits (Hank's buffer). Further biodegradation in NCS, pancreatin and synthetic juice was characterized by significant surface and bulk erosion. Only in pancreatin and NCS did biodegradation proceed to a loss of spherical shape and partial (pancreatin) and almost total (NCS) disruption of microcapsule structure after 30 days.
T W Atkins; S J Peacock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microencapsulation     Volume:  14     ISSN:  0265-2048     ISO Abbreviation:  J Microencapsul     Publication Date:    1997 Jan-Feb
Date Detail:
Created Date:  1997-04-02     Completed Date:  1997-04-02     Revised Date:  2009-07-21    
Medline Journal Info:
Nlm Unique ID:  8500513     Medline TA:  J Microencapsul     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  35-49     Citation Subset:  IM    
Department of Pharmaceutical and Biological Sciences, Aston University, Birmingham, UK.
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MeSH Terms
Animals, Newborn
Biodegradation, Environmental
Gastric Juice / chemistry*
Indicators and Reagents
Microscopy, Electron, Scanning
Pancreatin / chemistry*
Polyesters / chemistry*
Reg. No./Substance:
0/Buffers; 0/Capsules; 0/Indicators and Reagents; 0/Polyesters; 0/poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); 8049-47-6/Pancreatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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